Scopus
YÖKSİS Eşleşti
A novel conductometric creatinine biosensor based on solid-state contact ammonium sensitive PVC-NH <inf>2</inf> membrane
Biochemical Engineering Journal · Mart 2012
YÖKSİS Kayıtları
A novel conductometric creatinine biosensor based on solid state contact ammonium sensitive PVC NH2 membrane
Biochemical Engineering Journal · 2012 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ VİLDAN ERCİ →
Makale Bilgileri
DergiBiochemical Engineering Journal
Yayın TarihiMart 2012
Cilt / Sayfa62 · 34-38
Scopus ID2-s2.0-84856576845
Özet
A novel, highly sensitive and stable conductometric biosensor for creatinine determination is developed. The biosensor is based on solid-state contact ammonium-sensitive sensor. Creatininase is chemically immobilized on the surface of the solid-state contact ammonium-sensitive membrane via glutaraldehyde covalent attachment method. The developed conductometric creatinine biosensors demonstrate high sensitivity and short response time toward creatinine. The detection limit of the biosensor was about 2×10 -6M and the response time was shorter than 10s in phophate buffer solution at pH 7.20. The linear dynamic range of the biosensor was between 1×10 -1 and 9×10 -6M creatinine concentration in phosphate buffer solution at pH 7.20. The biosensor exhibited good operational and storage stability for at least 4 weeks kept in dry at 4-6°C. It had a reproducible and stable response during continuous work at least for 10h with the relative standard deviation of 0.5% (n=48) for creatinine of 1×10 -3M in phosphate buffer solution. © 2011 Elsevier B.V..
Yazarlar (6)
1
Ibrahim Isildak
2
Osman Cubuk
3
Melda Altikatoglu
4
Murat Yolcu
5
Vildan Erci
6
Nihat Tinkilic
Anahtar Kelimeler
Biosensor
Conductometric biosensor chip
Creatininase immobilization
Creatinine
Solid-state biosensor
Kurumlar
Ondokuz Mayis Üniversitesi
Samsun Turkey
Yıldız Teknik Üniversitesi
Istanbul Turkey
Metrikler
26
Atıf
6
Yazar
5
Anahtar Kelime