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Scopus 🔓 Açık Erişim YÖKSİS ISSN Eşleşti SJR Q3

Biomarkers to target and silence stemness of breast cancer stem cell model: silencing MDR1 by siRNA

Turkish Journal of Biochemistry · Ağustos 2022

YÖKSİS ISSN Eşleşmesi

Bu dergide (ISSN eşleşmesi) kurumun 20 kaydı bulundu.

YÖKSİS Kayıtları — ISSN Eşleşmesi
Asymmetric dimethylarginine and arginine levels in patients with rheumatoid arthritis
2013 ISSN: 0250-4685 SCI-Expanded
Prof. Dr. ALİ ÜNLÜ →
Asymmetric dimethylarginine and arginine levels in patients with rheumatoid arthritis
2013 ISSN: 0250-4685 SCI-Expanded 3 atıf
Prof. Dr. ALİ ÜNLÜ →
Asymmetric dimethylarginine levels in Behçet s Disease
2014 ISSN: 0250-4685 SCI-Expanded
Prof. Dr. BAHADIR ÖZTÜRK →
Asymmetric dimethylarginine levels in Behçet s Disease
2014 ISSN: 0250-4685 SCI-Expanded
Prof. Dr. HÜSAMETTİN VATANSEV →
Asymmetric dimethylarginine and arginine levels in patients with rheumatoid arthritis
2013 ISSN: 0250-4685 SCI-Expanded 3 atıf
Prof. Dr. HÜSAMETTİN VATANSEV →
Asymmetric dimethylarginine and arginine levels in patients with rheumatoid arthritis
2013 ISSN: 0250-4685 SCI-Expanded
Prof. Dr. ABDULLAH SİVRİKAYA →
Is median value of Double Screening Test regional?
2015 ISSN: 0250-4685 SCI-Expanded Q4
Doç. Dr. RAMAZAN KOCABAŞ →
Calculation of measurement uncertainty of biochemical parameters
2015 ISSN: 02504685 SCI-Expanded
Prof. Dr. SEDAT ABUŞOĞLU →
The effects of grape seed extract against toxicity of benzene on liver and kidney tissues of albino mice biochemical evaluation
2015 ISSN: 0250-4685 SCI-Expanded
Prof. Dr. ERTAN ORUÇ →
Analysis of trace elements in epileptic patients
2015 ISSN: 0250-4685 SCI-Expanded
Prof. Dr. BAHADIR ÖZTÜRK →
Serum Paraoxonase Levels and Oxidative Stress in Patients with Scleroderma
2015 ISSN: 0250-4685 SCI-Expanded
Prof. Dr. HÜSAMETTİN VATANSEV →
Analysis of Trace Elements in Epileptic Patients
2015 ISSN: 0250-4685 SCI-Expanded
Prof. Dr. HÜSAMETTİN VATANSEV →
The Analytical Performance of The C Petide and Insuline Test
2015 ISSN: 0250-4685 SCI-Expanded
Prof. Dr. HÜSAMETTİN VATANSEV →
Procalcitonin Levels in Inflammatory Disease
2015 ISSN: 0250-4685 SCI-Expanded
Prof. Dr. HÜSAMETTİN VATANSEV →
Comparison of Routine Biochemical and Hormonal Values of Sleeve Gastrectomy Applied Patients
2015 ISSN: 0250-4685 SCI-Expanded
Prof. Dr. HÜSAMETTİN VATANSEV →
Comparison Of MPV WBC and Neutrophil Lymphocyte Ratio of Sleeve Gastrectomy Applied Patients
2015 ISSN: 0250-4685 SCI-Expanded
Prof. Dr. HÜSEYİN YILMAZ →
Influence of Pinealectomy on Hemogram Parameters of Rats Applied to Sepsis Model
2015 ISSN: 0250-4685 SCI-Expanded
Prof. Dr. HÜSAMETTİN VATANSEV →
Meme Kanser Hücrelerinin Fluıdıgm Dynamıc Array Kullanılarak Kantitatif miRNA Ekspresyaon Analizi
2015 ISSN: 0250-4685 SCI-Expanded
Prof. Dr. ALİ ÜNLÜ →
25 OH D3 ve 1 25 OH 2 D3 ün MCF 7 Meme Kanser Hücre Hattının Proliferasyonu Üzerine Kıyaslanması
2015 ISSN: 0250-4685 SCI-Expanded
Prof. Dr. ALİ ÜNLÜ →
The effect of single dose administration of nandrolone decanoate on plasma irisin adropin leptin levelsTek doz nandrolon dekonat uygulamasının plazma irisin adropin leptin düzeylerine etkisi
2015 ISSN: 0250-4685 SCI-Expanded
Prof. Dr. SEYFULLAH HALİLOĞLU →

Makale Bilgileri

Dergi Turkish Journal of Biochemistry
ISSN02504685
Yayın TarihiAğustos 2022
Cilt / Sayfa47 · 445-455
DOI10.1515/tjb-2021-0275
Scopus ID2-s2.0-85146395314
Erişim🔓 Açık Erişim
Özet Objectives: Aim of the study was to reveal new biomarker genes to target breast cancer stem-like cells (BCSC-like) and then sensitize BCSC-like cells to chemotherapy by silencing MDR1 gene found to be the most suitable target. Methods: Drug resistance associated genes were screened by cDNA microarray to unveil biomarker genes in drug resistance breast cancer model cells. Drug resistance was then reversed by silencing MDR1 gene in BCSC-like cells. The effect of silencing was monitored by real-time cell proliferation analysis. Differential expressions of MDR1, ALDH1A3, EGFR and BAG4 genes were identified by real-time PCR. P-glycoprotein (P-gp) expression level and its activity were investigated by Western blot and flow cytometry measurements, respectively. Results: 16 new biomarker genes were identified upon gene expression analysis by cDNA microarray. MDR1 gene was selected as the most potent target gene and silencing of it caused down-regulation of MDR1, ALDH1A3, EGFR, BAG4 expression and P-glycoprotein activity and expression in BCSC-like cells. At the end, silenced BCSC-like cells were found to be more responsive to paclitaxel therapy. Conclusions: In conclusion, siMDR1 silencing is an effective way to reverse multidrug resistance and malignancy. New biomarker genes revealed in this study require to be investigated to target stemness of BC.

Yazarlar (4)

1
Gamze Yıldırım
ORCID: 0000-0002-6764-7349
2
Meltem Demirel Kars
3
Gökhan Kars
ORCID: 0000-0002-2507-2305
4
Hamdi Şükür Kılıç

Anahtar Kelimeler

ALDH1A3 breast cancer stem cells EGFR microarray paclitaxel siMDR1

Kurumlar

Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Turkish Journal of Biochemistry
Q3 OA
SJR Skoru0,232
H-Index26
YayıncıWalter de Gruyter GmbH
ÜlkeGermany
Biochemistry (medical) (Q3)
Biochemistry (Q4)
Clinical Biochemistry (Q4)
Molecular Biology (Q4)
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Metrikler

1
Atıf
4
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