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Increased apoptosis, tumor necrosis factor-α, and DNA damage attenuated by 3',4'-dihydroxyflavonol in rats with brain İschemia-reperfusion

Indian Journal of Pharmacology · Ocak 2021

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YÖKSİS Kayıtları
Increased apoptosis, tumor necrosis factor-α, and DNA damage attenuated by 3',4'-dihydroxyflavonol in rats with brain İschemia-reperfusion
Indian Journal of Pharmacology · 2021 SCI-Expanded
PROFESÖR ENDER ERDOĞAN →
Increased apoptosis, tumor necrosis factor-α, and DNA damage attenuated by 3',4'-dihydroxyflavonol in rats with brain İschemia-reperfusion
Indian Journal of Pharmacology · 2021 SCI-Expanded
PROFESÖR RASİM MOĞULKOÇ →
Increased apoptosis, tumor necrosis factor-α, and DNA damage attenuated by 3',4'-dihydroxyflavonol in rats with brain İschemia-reperfusion
Indian Journal of Pharmacology · 2021 SCI-Expanded
PROFESÖR ABDULKERİM KASIM BALTACI →

Makale Bilgileri

DergiIndian Journal of Pharmacology
Yayın TarihiOcak 2021
Cilt / Sayfa53 · 39-49
Özet OBJECTIVES: This research was aimed to find out the effects of 3',4'-dihydroxyflavonol (DiOHF) on apoptosis, DNA damage, and tumor necrosis factor-α (TNF-α) levels in the frontal cortex of rats with induced experimental brain ischemi reperfusion. MATERIALS AND METHODS: A total of 38 Wistar albino male rats were used. Groups were created as 1-Sham; 2-Ischemia-reperfusion (I/R); 3-I/R + DiOHF (10 mg/kg); 4-Ischemia + DiOHF + reperfusion; 5-DiOHF + I/R. I/R was performed by carotid artery ligation for 30 min in anesthesized animals. Following experimental applications, blood samples were taken from anesthetized rats to obtain erythrocyte and plasma. Later, the rats were killed by cervical dislocation, and frontal cortex samples were taken and stored at - 80oC for the analysis. RESULTS: In the ischemic frontal cortex tissue sections degenerate neuron numbers, Terminal deoxynucleotidyl transferase-dUTP nick end labeling (TUNEL) positive cell ratio and caspase-3 positive cell ratio increased. Malondialdehyde, TNF-α, and 8-OHdG levels were increased in both plasma and tissue in ischemia group, whereas tissue and erythrocyte glutathione levels were significantly suppressed. However, these values were significantly reversed by DiOHF treatment. CONCLUSION: The results of the study showed that I/R significantly increased apoptosis, TNF-α, and DNA damage in rats with brain I/R. However, 10 mg/kg intraperitoneal DiOHF treatment improved deterioted parameters.

Yazarlar (6)

1
Dervis Dasdelen
2
Merve Solmaz
3
Esma Menevşe
4
Rasim Mogulkoc
5
Abdulkerim Kasim Baltaci
6
Ender Erdoğan

Anahtar Kelimeler

3' 4'-Dihydroxyflavonol 8-OHdG brain ischemia-reperfusion caspase-3 tumor necrosis factor-α

Kurumlar

Selçuk Tip Fakültesi
Konya Turkey

Metrikler

9
Atıf
6
Yazar
6
Anahtar Kelime

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