Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
The effects of resveratrol and melatonin on biochemical and molecular parameters in diabetic old female rat hearts
Experimental Gerontology · Şubat 2023
YÖKSİS Kayıtları
The effects of resveratrol and melatonin on biochemical and molecular parameters in diabetic old female rat hearts.
Experimental gerontology · 2023 SCI-Expanded
PROFESÖR RASİM MOĞULKOÇ →
Makale Bilgileri
DergiExperimental Gerontology
Yayın TarihiŞubat 2023
Cilt / Sayfa172
Scopus ID2-s2.0-85143966968
Erişim🔓 Açık Erişim
Özet
The roles of melatonin and resveratrol-enhanced activation of SIRT1 (silent information regulator 1), GLUT4 (glucose transporter type 4), and PGC-1α (peroxisome proliferator-activated receptor gamma coactivator 1-alpha) in mediating the protective effects on the heart in aged female rats with streptozotocin-induced diabetes were investigated. 16-month-old 48 Wistar female rats were separated into 8 groups with equal numbers. Group 1: Control, Group 2: Resveratrol Control, Group 3: Melatonin Control, Group 4: Resveratrol and Melatonin Control, Group 5: Diabetes, Group 6: Diabetes Resveratrol, Group 7: Diabetes Melatonin, Group 8: Diabetes Resveratrol and Melatonin. A single dose of 40 mg/kg intraperitoneal streptozotocin was injected into the rats of Groups 5, 6, 7, and 8 to induce experimental diabetes. Blood glucose levels were measured from the tail veins of the animals six days after the injections, using a diagnostic glucose kit. Rats with a blood glucose levels ≥300 mg/dl were considered diabetic. 5 mg/kg/day of resveratrol (intraperitoneal) and melatonin (subcutaneous) were administered for four weeks. At the end of the applications, SIRT1, GLUT4, PGC-1α gene expression as well as MDA and GSH levels in the heart tissues were determined by the PCR method from heart tissue samples taken under general anesthesia. The findings of our study show that suppressed antioxidant activity and decreased GLUT4, SIRT1, and PGC-1α gene expression in heart tissue can be reversed by the combination of resveratrol, melatonin, and resveratrol + melatonin in a diabetic aged female rat model. Resveratrol and melatonin supplementation may have a protective effect on cardiac functions in the diabetic aged female rat model.
Yazarlar (6)
1
Nilufer Akgun-Unal
2
Serhan Ozyildirim
3
Omer Unal
4
Elif Gulbahce-Mutlu
5
Rasim Mogulkoc
6
Abdulkerim Kasim Baltaci
Anahtar Kelimeler
Aged female rat
Antioxidant
Diabetes
Melatonin
Resveratrol
Kurumlar
Istanbul University-Cerrahpasa
Istanbul Turkey
Karatay Üniversitesi
Konya Turkey
Kirikkale Üniversitesi
Kirikkale Turkey
Ondokuz Mayis University, Medical School
Samsun Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
5
Atıf
6
Yazar
5
Anahtar Kelime