Scopus
YÖKSİS Eşleşti
Ozone Prevents Cochlear Damage From Ischemia–Reperfusion Injury in Guinea Pigs
Artificial Organs · Ağustos 2017
YÖKSİS Kayıtları
Ozone Prevents Cochlear Damage From Ischemia-Reperfusion Injury in Guinea Pigs
Artificial Organs · 2017 SCI-Expanded
PROFESÖR JALE BENGİ ÇELİK →
Ozone Prevents Cochlear Damage From Ischemia–Reperfusion Injury in Guinea Pigs
ARTIFICIAL ORGANS · 2016 SCI-Expanded
PROFESÖR JALE BENGİ ÇELİK →
Ozone Prevents Cochlear Damage From Ischemia-Reperfusion Injury in Guinea Pigs
Artificial Organs · 2017 SSCI
DOÇENT ÇAĞDAŞ ELSÜRER →
Ozone Prevents Cochlear Damage From Ischemia-Reperfusion Injury in Guinea Pigs
Artificial Organs · 2017 SCI
PROFESÖR ENDER ERDOĞAN →
Ozone Prevents Cochlear Damage From Ischemia–Reperfusion Injury in Guinea Pigs
ARTIFICIAL ORGANS · 2016 SCI-Expanded
DOÇENT ÇAĞDAŞ ELSÜRER →
Ozone Prevents Cochlear Damage From Ischemia–Reperfusion Injury in Guinea Pigs
ARTIFICIAL ORGANS · 2016 SCI-Expanded
DOÇENT MERİH ÖNAL →
Ozone Prevents Cochlear Damage From Ischemia-Reperfusion Injury in Guinea Pigs
Artificial Organs · 2017 SSCI
PROFESÖR İBRAHİM ÖZKAN ÖNAL →
Makale Bilgileri
DergiArtificial Organs
Yayın TarihiAğustos 2017
Cilt / Sayfa41 · 744-752
Scopus ID2-s2.0-85014395408
Özet
The cochlea is an end organ, which is metabolically dependent on a nutrient and oxygen supply to maintain its normal physiological function. Cochlear ischemia and reperfusion (IR) injury is considered one of the most important causes of human idiopathic sudden sensorineural hearing loss. The aim of the present study was to study the efficacy of ozone therapy against cochlear damage caused by IR injury and to investigate the potential clinical use of this treatment for sudden deafness. Twenty-eight guinea pigs were randomized into four groups. The sham group (S) (n = 7) was administered physiological saline intraperitoneally (i.p.) for 7 days. The ozone group (O) (n = 7) was administered 1 mg/kg of ozone i.p. for 7 days. In the IR + O group (n = 7), 1 mg/kg of ozone was administered i.p. for 7 days before IR injury. On the eighth day, the IR + O group was subjected to cochlear ischemia for 15 min by occluding the bilateral vertebral artery and vein with a nontraumatic clamp and then reperfusion for 2 h. The IR group was subjected to cochlear IR injury. After the IR procedure, the guinea pigs were sacrificed on the same day. In a general histological evaluation, cochlear and spiral ganglionic tissues were examined with a light microscope, and apoptotic cells were counted by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. The apoptotic index (AI) was then calculated. Blood samples were sent for analyses of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase, malondialdehyde (MDA), the total oxidant score (TOS), and total antioxidant capacity (TAC). Data were evaluated statistically using the Kruskal–Wallis test. The AI was highest in the IR group. The AI of the IR + O group was lower than that of the IR group. The biochemical antioxidant parameters SOD and GSH-Px and the TAC values were highest in the O group and lowest in the IR group. The MDA level and TOS were highest in the IR group and lowest in the O group. Controlled ozone administration stimulated endogenous antioxidant defense systems, thereby helping the body to combat IR injury. Although this study revealed a statistically significant decrease in cochlear IR damage following ozone therapy, further studies will be necessary to explain the protective mechanisms of ozone therapy in cochlear IR injury.
Yazarlar (8)
1
Merih Onal
ORCID: 0000-0003-0591-8411
2
Çağdaş Elsürer
ORCID: 0000-0001-9804-7023
3
Nebil Selimoglu
4
Mustafa Yilmaz
5
Ender Erdoğan
6
Jale Bengi Celik
7
Oznur Kal
8
Ozkan Onal
ORCID: 0000-0002-5574-1901
Anahtar Kelimeler
Apoptosis
Cochlea
Guinea pigs
Ischemia–reperfusion injury
Ozone
Kurumlar
Başkent Üniversitesi
Ankara Turkey
Konya Training and Research Hospital
Konya Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
20
Atıf
8
Yazar
5
Anahtar Kelime