Scopus
🔓 Açık Erişim
Identification of genetic variation in the major histocompatibility complex gene region in Turkish sheep breeds
South African Journal of Animal Science · Ocak 2016
Makale Bilgileri
DergiSouth African Journal of Animal Science
Yayın TarihiOcak 2016
Cilt / Sayfa46 · 366-372
Scopus ID2-s2.0-85007395856
Erişim🔓 Açık Erişim
Özet
The major histocompatibility complex (MHC) in sheep, Ovar-Mhc, remains poorly characterized relative to other domestic animals. However, its basic structure is similar to that of other mammals, comprising class I, II and III regions. In this study, the Ovine MHC class II DRB1 and DRB3 genes were amplified by polymerase chain reaction in eight sheep breeds reared in Turkey. Informative restriction fragment length polymorphisms (RFLPs) were obtained with five restriction enzymes for DRB1 and with two restriction enzymes for DRB3. The digestion of DRB1 exon 2 with NciI, SacI, SacII, Hin1I each produced three genotypes and two alleles (viz., a and b) with frequencies of 0.69 and 0.31; 0.65 and 0.35; 0.91 and 0.09; 0.57 and 0.43, respectively. The digestion of DRB1 exon 2 with DdeI produced four genotypes and three alleles (viz., a, b and c) with frequencies of 0.62, 0.28 and 0.10, respectively. On the other hand, the digestion of DRB3 exon 2 with NdeII and BsaI each produced three genotypes and two alleles (viz., a and b) with frequencies of 0.72 and 0.28; 0.96 and 0.04, respectively. This study presents the genetic profiles of the exon 2 region of the MHC DRB1 and DRB3 genes in native Turkish sheep breeds.
Yazarlar (3)
1
Fatma Ilhan
2
İsmail Keskin
3
A. Tozluca
Anahtar Kelimeler
DRB1
DRB3
Polymerase chain reaction (PCR)
Restriction fragment length polymorphism (RFLP)
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
3
Atıf
3
Yazar
4
Anahtar Kelime