Scopus
YÖKSİS Eşleşti
Revealing the proteolytic characteristics of lactobacillus, lacticaseibacillus, and lactiplantibacillus isolates by in vitro and in situ perspectives
Food Bioscience · Ekim 2023
YÖKSİS Kayıtları
Revealing the proteolytic characteristics of lactobacillus, lacticaseibacillus, and lactiplantibacillus isolates by in vitro and in situ perspectives
Elsevier BV · 2023 SCI-Expanded
DOÇENT TALHA DEMİRCİ →
Revealing the proteolytic characteristics of lactobacillus, lacticaseibacillus, and lactiplantibacillus isolates by in vitro and in situ perspectives
Elsevier BV · 2023 SCI-Expanded
PROFESÖR NİHAT AKIN →
Makale Bilgileri
DergiFood Bioscience
Yayın TarihiEkim 2023
Cilt / Sayfa55
Scopus ID2-s2.0-85169610513
Özet
This study examined in vitro and in situ proteolytic characteristics of 8 lactobacilli strains isolated from Turkish Tulum cheese, consisting of L. plantarum, L. helveticus, L. rhamnosus, and L. paracasei, whose beneficial properties and safety concerns previously evaluated. The studied isolates presented versatile proteolytic activity with acidic, neutral, and alkaline proteases. The proteolytic activity of isolates against azocasein substrate was determined between 10.30 and 19.90 U/mg, and L. plantarum isolates had the highest proteolytic activity at varying pH values. Isolates 15, 449, 480 and 675 harboured proteinase genes in the catalytic region of prtR and prtP, and in the prtP/prtM intergenic region. All isolates were also evaluated in the production of fermented milk. As a result of SDS-PAGE analysis of fermented milk samples, L. plantarum isolates 449, 480 and 675 showed more proteolytic activity on milk caseins. Besides, isolate 480 was also effective in hydrolysis of beta-lactoglobulin. L. plantarum isolates resulted in an increase in amino acid (AA) concentrations especially in arginine, alanine, histidine, and proline during the 24-h fermentation of milk. AA production abilities varied depending on the strain. Generally, isolate 675 offered superior AA production ability. The proteolytic properties of cultures are effective on both human health and technological properties of the product in terms of reducing the allergenicity of milk proteins, forming functional peptides, and improving the sensory properties of the product. Based on the results of this study, L. plantarum isolates 449, 480 and 675 could potentially be used as starter cultures or co-cultures for these purposes.
Yazarlar (5)
1
Meryem Kübra Satılmış
2
Hale İnci Öztürk
ORCID: 0000-0001-8334-0403
3
Talha Demirci
4
Begüm Denktaş
5
Nihat Akın
ORCID: 0000-0002-0966-1126
Anahtar Kelimeler
Amino acid profile
Extracellular enzyme
Fermented milk
L. plantarum
Proteinase genes
Protein hydrolysis
Kurumlar
Konya Gida ve Tarim Üniversitesi
Konya Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
5
Atıf
5
Yazar
6
Anahtar Kelime