Scopus
🔓 Açık Erişim YÖKSİS DOI Eşleşti
SJR Q3
Prevalence, isolation and molecular characterization of Bovine Ephemeral Fever Virus in south and southeast regions of Turkey in the outbreak of 2020
Journal of the Hellenic Veterinary Medical Society · Ocak 2023
YÖKSİS Kayıtları
Prevalence, isolation and molecular
characterization of Bovine Ephemeral Fever Virus in
south and southeast regions of Turkey in the
outbreak of 2020
Journal of the Hellenic Veterinary Medical Society · 2023 CABI
Prof. Dr. OĞUZHAN AVCI →
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Makale Bilgileri
ISSN17922720
Yayın TarihiOcak 2023
Cilt / Sayfa74 · 6543-6552
Scopus ID2-s2.0-85187953321
Erişim🔓 Açık Erişim
Özet
The aim of this study is to investigate the presence of Bovine Ephemeral Fever (BEF) virus in EDTA-treated blood, sera, and spleen samples of 228 suspected animals originated from 5 different provinces of Turkey in 2020, as well as to perform molecular characterization and phylogenetic analysis of positive cases. For this purpose, 79 whole blood and 2 spleen samples as well as 168 sera samples from cattle varying in breed, age, and sex,were examined by using Real Time RT-PCR and Blocking ELISA, respectively. Two new degenerate primers amplifying 956 bp of the protein G (AVKEF_AATGTTCCNGTGAATTGTGGAG and AVKER_TGCATAATCYCTTCCTGGTCT) were designed for RT-PCR testing and the phylogenetic analysis of positive samples was performed. 64.20% (52/81) of the defibrinated blood (63.29%, 50/79) and spleen (100%, 2/2) samples, and 69.04% (116/168) of sera samples resulted positive by RT-PCR. By using VERO cell culture, BEF virus was isolated from blood (n=1) and spleen(n=2) tissues in Adana and Şanliurfa provinces. The phylogenetic analysis revealed that the BEF virus circulated in Turkey during the 2020 regional epidemic belongs to the Middle East lineage, which has its significance for the selection of proper vaccine and the control of the disease.
Yazarlar (5)
1
B. S. Tokgoz
2
E. A. Tokgoz
3
M. A. Sozmen
4
Oğuzhan Avci
ORCID: 0000-0001-9299-4695
5
A. E. Utuk
Anahtar Kelimeler
Bovine Ephemeral Fever
phylogenetics
real time RT-PCR
Turkey
virus isolation
Kurumlar
Adana Veterinary Control Institute
Adana Turkey
Çukurova Üniversitesi
Adana Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Journal of the Hellenic Veterinary Medical Society
Q3
SJR Skoru0,200
H-Index18
YayıncıHellenic Veterinary Medical Society
ÜlkeGreece
Veterinary (miscellaneous) (Q3)
Metrikler
2
Atıf
5
Yazar
5
Anahtar Kelime