Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Estimating genetic diversity among selected wild grapevine genotypes from Southern Turkey by simple sequence repeat (SSR) and inter-Primer Binding Site(iPBS) markers
Genetic Resources and Crop Evolution · Ocak 2024
YÖKSİS Kayıtları
Estimating genetic diversity among selected wild grapevine genotypes from Southern Turkey by simple sequence repeat (SSR) and inter-Primer Binding Site(iPBS) markers
Genetic Resources and Crop Evolution · 2024 SCI-Expanded
PROFESÖR ALİ SABIR →
Makale Bilgileri
DergiGenetic Resources and Crop Evolution
Yayın TarihiOcak 2024
Scopus ID2-s2.0-85200266227
Erişim🔓 Açık Erişim
Özet
The examination of germplasm within grapevine accessions derived from wild genotypes holds significant importance within the grapevine breeding program, particularly in the improving of cultivars and rootstocks. Due to the greater genetic variation present in wild genotypes, there is an increased possibility of possessing the desired features. The objective of this study was to determine the genetic diversity and population genetic structure of 64 grapevine genotypes using inter-primer binding site (iPBS) retrotransposon markers and simple sequence repeat (SSR) markers. A total of 236 bands were generated using iPBS markers, of which 162 bands exhibited polymorphism. A comprehensive assessment was conducted on a total of 126 SSR alleles using the SSR markers, revealing that 91 of these exhibited polymorphisms. Despite the similarity in mean values between polymorphic bands generated by iPBS (6.48) and SSR markers (6.5), the iPBS markers exhibited a greater polymorphism information content (PIC: 0.39) in comparison to SSR markers (0.29). The UPGMA analysis classified the genotypes into two primary groups at a similarity index of 0.62 based on combined data. The rootstocks utilized as points of reference are consolidated inside a singular cluster (A), distinct from both the Mediterranean wild population and cultivars. The comparison of genetic variation, represented by FST values, revealed that the maximum differentiation was observed between subpopulations SP3 and SP5. The wild grapevine population grown in the Southeast Mediterranean Region of Turkey exhibited significant differentiation. Both marker systems employed in this study were highly polymorphic and useful for genetic characterization and mapping of grapevine populations.
Yazarlar (5)
1
Hatice Ikten
ORCID: 0000-0002-7523-2039
2
Duygu Sari
3
Ali Sabir
ORCID: 0000-0003-1596-9327
4
Hasan Meydan
5
Nedim Mutlu
Anahtar Kelimeler
Genetic resources
Microsatellite
Molecular characterization
Retrotransposons
Vitis
Kurumlar
Akdeniz Üniversitesi
Antalya Turkey
Selçuk Üniversitesi
Selçuklu Turkey