Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Formulation of hesperidin-loaded in situ gel for ocular drug delivery: a comprehensive study
Journal of the Science of Food and Agriculture · Ağustos 2024
YÖKSİS Kayıtları
Formulation of hesperidin-loaded in situ gel for ocular drug delivery: a comprehensive study
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE · 2024 SCI
DOKTOR ÖĞRETİM ÜYESİ YAKUP GÜLTEKİN →
Makale Bilgileri
DergiJournal of the Science of Food and Agriculture
Yayın TarihiAğustos 2024
Cilt / Sayfa104 · 5846-5859
Scopus ID2-s2.0-85188449159
Erişim🔓 Açık Erişim
Özet
BACKGROUND: Allergic conjunctivitis is one of the most common eye disorders. Different drugs are used for its treatment. Hesperidin is an active substance isolated from Citrus sinensis L. (Rutaceae) fruit peels, with known anti-inflammatory activity but low solubility. It was complexed with cyclodextrin and encapsulated in situ gel to extend its duration in the eye. RESULTS: The optimized formulation comprised 1% hesperidin, 1.5% hydroxyethyl cellulose, and 16% poloxamer 407. The viscosity at 25 °C was 492 ± 82 cP, and at 35 °C it was 8875 ± 248 cP, the pH was 7.01 ± 0.03, gelation temperature was 34 ± 1.3 °C, and gelation time was 33 ± 1.2 s. There was a 66% in vitro release in the initial 2 h, with a burst effect. A lipoxygenase (LOX) inhibition test determined that hesperidin was active at high doses on leukotyrens seen in the body in allergic diseases. In cell-culture studies, the hesperidin cyclodextrin complex loaded in situ gel, BRN9-CD (poloxamer 16%, hydroxy ethyl cellulose (HEC) 1.5%), enhanced cell viability in comparison with the hesperidin solution. It was determined that BRN9-CD did not cause any irritation in the ocular tissues in the Draize test. CONCLUSION: The findings of this study demonstrate the potential of the in situ gel formulation of hesperidin in terms of ease of application and residence time on the ocular surface. Due to its notable LOX inhibition activity and positive outcomes in the in vivo Draize test, it appears promising for incorporation into pharmaceutical formulations. © 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Yazarlar (12)
1
Sefa Gözcü
ORCID: 0000-0002-0735-4229
2
Heybet Kerem Polat
3
Yakup Gultekin
4
Sedat Ünal
5
Nasıf Fatih Karakuyu
6
Esra Köngül Şafak
7
Osman Doğan
8
Esra Pezik
9
Muhammet Kerim Haydar
10
Eren Aytekin
11
Nihat Kurt
12
Burak Batuhan Laçin
Anahtar Kelimeler
cell viability
cyclodextrin
Draize test
hesperidin
lox inhibition
Kurumlar
Atatürk Üniversitesi
Erzurum Turkey
Erciyes Üniversitesi
Kayseri Turkey
Erzincan Binali Yıldırım Üniversitesi
Erzincan Turkey
Hacettepe Üniversitesi
Ankara Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Süleyman Demirel Üniversitesi
Isparta Turkey
T.C. Sağlık Bakanlığı,
Ankara Turkey
Tokat Gaziosmanpaşa Üniversitesi
Tokat Turkey
Metrikler
2
Atıf
12
Yazar
5
Anahtar Kelime