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In Vitro Analysis of the Co-Assembly of Type-I and Type-III Collagen

Cellular and Molecular Bioengineering · Şubat 2017

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YÖKSİS Kayıtları
In Vitro Analysis of the Co Assembly of Type I and Type III Collagen
Cellular and Molecular Bioengineering · 2016 SCI-Expanded
DOÇENT ESMA ERYILMAZ DOĞAN →

Makale Bilgileri

DergiCellular and Molecular Bioengineering
Yayın TarihiŞubat 2017
Cilt / Sayfa10 · 41-53
Özet An important step towards achieving functional diversity of biomimetic surfaces is to better understand the co-assembly of the extracellular matrix components. For this, we study type-I and type-III collagen, the two major collagen types in the extracellular matrix. By using atomic force microscopy, custom image analysis, and kinetic modeling, we study their homotypic and heterotypic assembly. We find that the growth rate and thickness of heterotypic fibrils decrease as the fraction of type-III collagen increases, but the fibril nucleation rate is maximal at an intermediate fraction of type-III. This is because the more hydrophobic type-I collagen nucleates fast and grows in both longitudinal and lateral directions, whereas more hydrophilic type-III limits lateral growth of fibrils, driving more monomers to nucleate additional fibrils. This demonstrates that subtle differences in physico-chemical properties of similar molecules can be used to fine-tune their assembly behavior.

Yazarlar (3)

1
Esma Eryilmaz Dogan
2
Winfried Teizer
3
Wonmuk Hwang

Anahtar Kelimeler

AFM Biomimetic surface Collagen Extracellular matrix Heterogeneous assembly Macromolecular assembly

Kurumlar

Korea Institute for Advanced Study
Seoul South Korea
Selçuk Üniversitesi
Selçuklu Turkey
Texas A&M University
College Station United States
Tohoku University
Sendai Japan

Metrikler

9
Atıf
3
Yazar
6
Anahtar Kelime

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