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Lycopene and resveratrol improve post-thaw bull sperm parameters: Sperm motility, mitochondrial activity and DNA integrity

Andrologia · Haziran 2015

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YÖKSİS Kayıtları
Lycopene and resveratrol improve post thaw bull sperm parameters sperm motility mitochondrial activity and DNA integrity
Andrologia · 2015 SCI-Expanded
PROFESÖR MUSTAFA NUMAN BUCAK →
Lycopene and resveratrol improve post thaw bull sperm parameters sperm motility mitochondrial activity and DNA integrity
Andrologia · 2015 SCI-Expanded
PROFESÖR NURİ BAŞPINAR →
Lycopene and resveratrol improve post thaw bull sperm parameters sperm motility mitochondrial activity and DNA integrity
Andrologia · 2015 SCI-Expanded
PROFESÖR NURİ BAŞPINAR →

Makale Bilgileri

DergiAndrologia
Yayın TarihiHaziran 2015
Cilt / Sayfa47 · 545-552
Erişim🔓 Açık Erişim
Özet We focussed on evaluating the protective effect of lycopene and resveratrol on post-thaw bull sperm and oxidative stress parameters. Nine ejaculates for each bull were used in the study. Each ejaculate, splitted into three equal aliquots and diluted at 37 °C with base extenders containing lycopene (1 × 10-3 g ml-1) and resveratrol (1 mm), and no antioxidant (control), was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. The supplementation of the semen extender with lycopene and resveratrol increased the percentages of post-thawed computer-assisted sperm analysis (CASA) motility (55.8 ± 3.8 and 61.9 ± 4.0%) and progressive motility (38 ± 2.4 and 37 ± 8.8), compared with the controls (50.7 ± 2.65 and 33.3 ± 3.74%, respectively, P < 0.05). Resveratrol provided a higher ALH (4.3 ± 0.1), in comparison with the control (3.9 ± 0.3, P < 0.05). The supplementation of the semen extender with lycopene and resveratrol produced a higher mitochondrial activity (24.6 ± 2.9 and 30.1 ± 6.5% respectively), compared with that of the control (11.8 ± 9.5%, P < 0.05). It was determined that both antioxidants resulted in a lower percentage of sperm with damaged DNA than that of the control (P < 0.05). Sperm motion characteristics except for ALH, acrosome integrity, sperm viability and oxidative stress parameters were not affected by the adding of lycopene and resveratrol.

Yazarlar (10)

1
Mustafa Numan Bucak
2
M. B. Ataman
3
N. Baspinar
4
O. Uysal
5
M. Taşpinar
6
A. Bilgili
7
Caner Ozturk
8
Şükrü Güngör
9
Muhammed Enes Inanç
10
E. Akal

Anahtar Kelimeler

Antioxidants Cryopreservation DNA damage Oxidative stress Sperm

Kurumlar

Ankara Üniversitesi
Ankara Turkey
Ondokuz Mayis Üniversitesi
Samsun Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Van Yüzüncü Yıl Üniversitesi
Van Turkey

Metrikler

83
Atıf
10
Yazar
5
Anahtar Kelime