Scopus
YÖKSİS Eşleşti
Effects of arginine and trehalose on post-thawed bovine sperm quality
Acta Veterinaria Hungarica · Eylül 2017
YÖKSİS Kayıtları
Effects of arginine and trehalose on post-thawed bovine sperm quality
Acta Veterinaria Hungarica · 2017 SCI
PROFESÖR NURİ BAŞPINAR →
Effects of Arginine and Trehalose on Post-thawed Bovine Sperm Quality
Acta Veterinaria Hungarica · 2017 SCI
PROFESÖR MUSTAFA NUMAN BUCAK →
Makale Bilgileri
DergiActa Veterinaria Hungarica
Yayın TarihiEylül 2017
Cilt / Sayfa65 · 429-439
Scopus ID2-s2.0-85030538986
Özet
The present study was conducted to examine the protective role of arginine and trehalose on post-thaw bull sperm and oxidative stress parameters. Five ejaculates for each bull were used in the study. Each ejaculate, split into three equal aliquots and diluted at 37 °C with base extenders containing 2 mM arginine, 25 mM trehalose and no antioxidant (control) was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. Supplementation of the semen extender with arginine decreased the percentages of post-thawed subjective motility (29 ± 8.21%), CASA motility (12.2 ± 5.69%) and progressive motility (3.52 ± 2.13%), compared with the controls (43 ± 2.73%, 55.4 ± 6.78% and 33.48 ± 4.14%, respectively, P < 0.05). Supplementation of the semen extender with trehalose produced a higher mitochondrial activity and sperm viability (36.3 ± 3.99% and 44.1 ± 2.18%) compared with the control (13 ± 8.15 and 31.7 ± 3.94%, respectively, P < 0.05). It was established that trehalose (95.1%) and arginine (92.8%) protect DNA integrity compared to the control (90.4%) (P < 0.05). Trehalose supplementation in semen extenders provided great benefit in terms of viability, mitochondrial activity, and intact sperm DNA on frozen-thawed bull sperm.
Yazarlar (7)
1
Caner Ozturk
2
Şükrü Güngör
3
M. B. Ataman
4
Mustafa Numan Bucak
5
N. Baspinar
6
Pinar Ili
7
Muhammed Enes Inanç
Anahtar Kelimeler
Arginine
Bull sperm
COMET
Fluorescent staining
Oxidative stress
Trehalose
Kurumlar
Aksaray Üniversitesi
Aksaray Turkey
Burdur Mehmet Akif Ersoy Üniversitesi
Burdur Turkey
Pamukkale Üniversitesi
Denizli Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
18
Atıf
7
Yazar
6
Anahtar Kelime