Scopus
🔓 Açık Erişim YÖKSİS DOI Eşleşti
SJR Q1
Local application of gingiva-derived mesenchymal stem cells on experimental periodontitis in rats
Journal of Periodontology · Mayıs 2024
YÖKSİS Kayıtları
Local application of gingiva‐derived mesenchymal stem cells on experimental periodontitis in rats
Wiley · 2023 SCI-Expanded
Dr. Öğr. Üyesi YUNUS EMRE BALABAN →
Local application of gingiva‐derived mesenchymal stem cells on experimental periodontitis in rats
JOURNAL OF PERIODONTOLOGY · 2023 SCI-Expanded
Dr. Öğr. Üyesi YUNUS EMRE BALABAN →
Local application of gingiva‐derived mesenchymal stem cells on experimental periodontitis in rats
Wiley · 2024 SCI-Expanded
Prof. Dr. SEMA HAKKI →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Boric Acid Irrigation as an Adjunct to Mechanical Periodontal Therapy in Patients With Chronic Periodontitis A Randomized Clinical Trial
2013 ISSN: 0022-3492 SCI-Expanded
Prof. Dr. UĞUR ARSLAN →
Comparison of Mesenchymal Stem Cells Isolated From Pulp and Periodontal Ligament
2015 ISSN: 0022-3492 SCI-Expanded
Prof. Dr. SEMA HAKKI →
Letter to the Editor Re Real Time Quantitative Polymerase Chain Reaction Analysis of Patients With Refractory Chronic Periodontitis
2013 ISSN: 0022-3492 SCI-Expanded
Prof. Dr. SEMA HAKKI →
Efficacy of Collagen Membrane Seeded With Autologous Gingival Fibroblasts in Gingival Recession Treatment A Randomized Controlled Pilot Study
2013 ISSN: 0022-3492 SCI-Expanded
Prof. Dr. SEMA HAKKI →
Boric Acid Irrigation as an Adjunct to Mechanical Periodontal Therapy in Patients With Chronic Periodontitis A Randomized Clinical Trial
2013 ISSN: 0022-3492 SCI-Expanded
Prof. Dr. SEMA HAKKI →
Bone Morphogenetic Protein 7 Enhances Cementoblast Function In Vitro
2010 ISSN: 0022-3492 SCI-Expanded 18 atıf
Prof. Dr. SEMA HAKKI →
Comparison of Er Cr YSGG Laser and Hand Instrumentation on the Attachment of Periodontal Ligament Fibroblasts to Periodontally Diseased Root Surfaces An In Vitro Study
2010 ISSN: 0022-3492 SCI-Expanded 15 atıf
Prof. Dr. SEMA HAKKI →
Comparison of Mesenchymal Stem Cells Isolated From Pulp and Periodontal Ligament
2015 ISSN: 0022-3492 SCI-Expanded
Prof. Dr. ERDOĞAN EŞREF HAKKI →
Dexamethasone and Basic Fibroblast Growth Factor Regulate Markers of Mineralization in Cementoblasts In Vitro
2005 ISSN: 0022-3492 SCI-Expanded
Prof. Dr. ERDOĞAN EŞREF HAKKI →
Bone Morphogenetic Protein 7 Enhances Cementoblast Function In Vitro
2010 ISSN: 0022-3492 SCI-Expanded
Prof. Dr. ERDOĞAN EŞREF HAKKI →
Letter to the Editor Re Real Time Quantitative Polymerase Chain Reaction Analysis of Patients With Refractory Chronic Periodontitis
2013 ISSN: 0022-3492 SCI-Expanded
Prof. Dr. ERDOĞAN EŞREF HAKKI →
Nicotine suppresses proliferation and mineralized tissue‐associated gene expressions of the cementoblasts
2019 ISSN: 0022-3492 SCI-Expanded
Prof. Dr. SEMA HAKKI →
Local application of gingiva‐derived mesenchymal stem cells on experimental periodontitis in rats
2024 ISSN: 0022-3492 SCI-Expanded Q1
Prof. Dr. SEMA HAKKI →
Differential effects of resolvin D1 and resolvin E1 on cementoblast function
2023 ISSN: 0022-3492 SCI-Expanded Q1
Prof. Dr. SEMA HAKKI →
Local application of gingiva‐derived mesenchymal stem cells on experimental periodontitis in rats
2023 ISSN: 0022-3492 SCI-Expanded Q1
Dr. Öğr. Üyesi YUNUS EMRE BALABAN →
Identification of the Difference in Extracellular Matrix and Adhesion Molecules of Cultured Human Gingival Fibroblasts Versus Juvenile Hyaline Fibromatosis Gingival Fibroblasts Using cDNA Microarray Analysis
2005 ISSN: 0022-3492 SCI
Prof. Dr. ERDOĞAN EŞREF HAKKI →
Makale Bilgileri
ISSN00223492
Yayın TarihiMayıs 2024
Cilt / Sayfa95 · 456-468
Scopus ID2-s2.0-85173525478
Erişim🔓 Açık Erişim
Özet
Background: Stem cell-based approaches in regenerative periodontal therapy have been used in different experimental models. In this study, the effect of local application of gingival mesenchymal stem cells (GMSC) in fibroin/chitosan oligosaccharide lactate hydrogel (F/COS) on periodontal regeneration was evaluated using experimental periodontitis model in rats. Methods: Mesenchymal stem cells were isolated from the gingiva of rats and characterized. Viability tests and confocal imaging of GMSC in hydrogels were performed. Healthy control without periodontitis (Health; H; n=10), control with periodontitis but no application (Periodontitis; P; n=10), only hydrogel application (F/COS; n=10), and GMSC+F/COS (n=10) four groups were formed for in vivo studies. Experimental periodontitis was created with silk sutures around the maxillary second molars. GMSC labeled with green fluorescent protein (GFP) (250,000 cells/50 μL) in F/COS were applied to the defect. Animals were sacrificed at 2nd and 8th weeks and maxillae of the animals were evaluated by micro-computed tomography (micro-CT) and histologically. The presence of GFP-labeled GMSC was confirmed at the end of 8 weeks. Results: Micro-CT analysis showed statistically significant new bone formation in the F/COS+GMSC treated group compared with the P group at the end of 8 weeks (p < 0.05). New bone formation was also observed in the F/COS group, but the statistical analysis revealed that this difference was not significant when compared with the P group (p > 0.05). Long junctional epithelium formation was less in the F/COS+GMSC group compared with the P group. Periodontal ligament and connective tissue were well-organized in F/COS+GMSC group. Conclusion: The results showed that local GMSC application in hydrogel contributed to the formation of new periodontal ligament and alveolar bone in rats with experimental periodontitis. Since gingiva is easly accessible tissue, it is promising for autologous cell-based treatments in clinical applications.
Yazarlar (9)
1
Yunus Emre Balaban
ORCID: 0000-0002-1754-4296
2
Sema Akbaba
3
Şerife Buket Bozkurt
4
Arda Buyuksungur
5
E. Ece Akgun
6
Zeynep Burcin Gonen
7
Hasan Salkin
8
Aysen Tezcaner
9
Sema S. Hakkı
Anahtar Kelimeler
hydrogel
mesenchymal stem cell
periodontal regeneration
periodontitis
Kurumlar
Afyon Kocatepe Üniversitesi
Afyonkarahisar Turkey
Ankara Üniversitesi
Ankara Turkey
Erciyes Üniversitesi
Kayseri Turkey
Middle East Technical University (METU)
Ankara Turkey
Niğde Ömer Halisdemir University
Nigde Turkey
Selçuk Üniversitesi
Selçuklu Turkey
T.C. Beykent Üniversitesi
Istanbul Turkey
Scimago Dergi (ISSN Eşleşmesi)
Journal of Periodontology
Q1
SJR Skoru1,324
H-Index186
YayıncıJohn Wiley & Sons Inc.
ÜlkeUnited States
Periodontics (Q1)
Metrikler
16
Atıf
9
Yazar
4
Anahtar Kelime