Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Local application of gingiva-derived mesenchymal stem cells on experimental periodontitis in rats
Journal of Periodontology · Mayıs 2024
YÖKSİS Kayıtları
Local application of gingiva‐derived mesenchymal stem cells on experimental periodontitis in rats
Wiley · 2023 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ YUNUS EMRE BALABAN →
Local application of gingiva‐derived mesenchymal stem cells on experimental periodontitis in rats
Wiley · 2024 SCI-Expanded
PROFESÖR SEMA HAKKI →
Local application of gingiva‐derived mesenchymal stem cells on experimental periodontitis in rats
JOURNAL OF PERIODONTOLOGY · 2023 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ YUNUS EMRE BALABAN →
Makale Bilgileri
DergiJournal of Periodontology
Yayın TarihiMayıs 2024
Cilt / Sayfa95 · 456-468
Scopus ID2-s2.0-85173525478
Erişim🔓 Açık Erişim
Özet
Background: Stem cell-based approaches in regenerative periodontal therapy have been used in different experimental models. In this study, the effect of local application of gingival mesenchymal stem cells (GMSC) in fibroin/chitosan oligosaccharide lactate hydrogel (F/COS) on periodontal regeneration was evaluated using experimental periodontitis model in rats. Methods: Mesenchymal stem cells were isolated from the gingiva of rats and characterized. Viability tests and confocal imaging of GMSC in hydrogels were performed. Healthy control without periodontitis (Health; H; n=10), control with periodontitis but no application (Periodontitis; P; n=10), only hydrogel application (F/COS; n=10), and GMSC+F/COS (n=10) four groups were formed for in vivo studies. Experimental periodontitis was created with silk sutures around the maxillary second molars. GMSC labeled with green fluorescent protein (GFP) (250,000 cells/50 μL) in F/COS were applied to the defect. Animals were sacrificed at 2nd and 8th weeks and maxillae of the animals were evaluated by micro-computed tomography (micro-CT) and histologically. The presence of GFP-labeled GMSC was confirmed at the end of 8 weeks. Results: Micro-CT analysis showed statistically significant new bone formation in the F/COS+GMSC treated group compared with the P group at the end of 8 weeks (p < 0.05). New bone formation was also observed in the F/COS group, but the statistical analysis revealed that this difference was not significant when compared with the P group (p > 0.05). Long junctional epithelium formation was less in the F/COS+GMSC group compared with the P group. Periodontal ligament and connective tissue were well-organized in F/COS+GMSC group. Conclusion: The results showed that local GMSC application in hydrogel contributed to the formation of new periodontal ligament and alveolar bone in rats with experimental periodontitis. Since gingiva is easly accessible tissue, it is promising for autologous cell-based treatments in clinical applications.
Yazarlar (9)
1
Yunus Emre Balaban
ORCID: 0000-0002-1754-4296
2
Sema Akbaba
3
Şerife Buket Bozkurt
4
Arda Buyuksungur
5
E. Ece Akgun
6
Zeynep Burcin Gonen
7
Hasan Salkin
8
Aysen Tezcaner
9
Sema S. Hakkı
Anahtar Kelimeler
hydrogel
mesenchymal stem cell
periodontal regeneration
periodontitis
Kurumlar
Afyon Kocatepe Üniversitesi
Afyonkarahisar Turkey
Ankara Üniversitesi
Ankara Turkey
Erciyes Üniversitesi
Kayseri Turkey
Middle East Technical University (METU)
Ankara Turkey
Niğde Ömer Halisdemir University
Nigde Turkey
Selçuk Üniversitesi
Selçuklu Turkey
T.C. Beykent Üniversitesi
Istanbul Turkey
Metrikler
5
Atıf
9
Yazar
4
Anahtar Kelime