Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Investigation of the Effects of Juglone-Selenium Treatments on Epithelial-Mesenchimal Transition and Migration in BxPC-3 and PANC-1 Human Pancreatic Cancer Cells
Medical Journal of Bakirkoy · Ocak 2023
YÖKSİS Kayıtları
Investigation of the Effects of Juglone-Selenium Treatments on Epithelial-Mesenchimal Transition and Migration in BxPC-3 and PANC-1 Human Pancreatic Cancer Cells
Bakırköy Tıp Dergisi · 2023 ESCI, EBSCO, Scopus, TR dizin
DOÇENT DUDU ERKOÇ KAYA →
Investigation of the Effects of Juglone-Selenium Treatments on Epithelial-Mesenchimal Transition and Migration in BxPC-3 and PANC-1 Human Pancreatic Cancer Cells
Galenos Yayinevi · 2023 ESCI
PROFESÖR HİLAL ARIKOĞLU →
Investigation of the Effects of Juglone-Selenium Treatments on Epithelial-Mesenchimal Transition and Migration in BxPC-3 and PANC-1 Human Pancreatic Cancer Cells
Bakırköy Tıp Dergisi · 2023 ESCI, EBSCO, Scopus, TR dizin
ARAŞTIRMA GÖREVLİSİ FATMA GÖKTÜRK →
Makale Bilgileri
DergiMedical Journal of Bakirkoy
Yayın TarihiOcak 2023
Cilt / Sayfa19 · 248-255
Scopus ID2-s2.0-85176738993
Erişim🔓 Açık Erişim
Özet
Objective: Chemotherapy is still the most common and primary treatment option for treating pancreatic cancers because metastasis has already occurred at the time of diagnosis, difficulties of the operation due to its location, and lack of targeted treatment approaches. Moreover, the high chemoteuropatic resistance seen in pancreatic cancer makes research on alternative drug treatments mandatory. Juglone, a natural naphthaquinone found in members of the Juglandaceae family, has various pharmacological effects such as antiviral and antibacterial. It has also been shown to be an effective cytotoxic agent through the production of reactive oxygen species in studies conducted with cancer cell lines. Remarkably, selenium, an important trace element of the cell, was shown to inhibit metastasis, strengthen cell-cell attachments, and reduce angiogenesis. Based on these data, we investigated the effects of juglone-selenium (J/S) combination on epithelial-mesenchymal transition (EMT) and migration in PANC-1 and BxPC-3 cells. Methods: The effects of juglone application in the presence of 2,5 µM NaSe at 5, 10, 15 and 20 µM concentrations on the expression of FOXL1, VIM, and MMP-7 genes were determined by qPCR. In addition, the effects of J/S on the migration features of cancer cells were monitored by wound healing tests. Results: According to qPCR results, J/S application showed different effects on FOXL1, VIM, and MMP-7 gene expressions, which are critical for EMT, in both cell lines. According to the wound healing assay results, the migration of cancer cells was suppressed in both cell lines compared with the control group. Conclusion: Consequently, our studies have supported that juglone can be an effective therapeutic agent on pancreatic cancer, and our findings also suggest that selenium can strengthen these anticancer effects of juglone.
Yazarlar (4)
1
Dudu Erkoc-Kaya
ORCID: 0000-0003-0114-6602
2
Fatma Gokturk
3
Fatma Batırbek
4
Hilal Arikoglu
ORCID: 0000-0002-6600-6603
Anahtar Kelimeler
epithelial-mesenchymal transition (EMT)
Juglone
pancreatic cancer
selenium
wound-healing assay
Kurumlar
Selçuk Tip Fakültesi
Konya Turkey
Metrikler
1
Atıf
4
Yazar
5
Anahtar Kelime