Scopus
YÖKSİS Eşleşti
Effects of Juglone and Curcumin Administration on Expression of FABP5 and FABP9 in MCF-7 and MDA-MB-231 Breast Cancer Cell Lines
Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology · Mart 2023
YÖKSİS Kayıtları
Effects of Juglone and Curcumin Administration on Expression of FABP5 and FABP9 in MCF-7 and MDA-MB-231 Breast Cancer Cell Lines
Biochemistry Moscow-Supplement Series A: Membrane and Cell Biology · 2023 SCI-Expanded
PROFESÖR ESMA MENEVŞE →
Effects of Juglone and Curcumin Administration on Expression of FABP5 and FABP9 in MCF-7 and MDA-MB-231 Breast Cancer Cell Lines
Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology · 2023 ESCI
DOÇENT DUDU ERKOÇ KAYA →
Makale Bilgileri
DergiBiochemistry (Moscow) Supplement Series A: Membrane and Cell Biology
Yayın TarihiMart 2023
Cilt / Sayfa17 · 58-67
Scopus ID2-s2.0-85151476488
Özet
Abstract—: Among natural chemopreventive agents, polyphenol curcumin and naphthoquinone juglone, which has broad-spectrum anticancer activity, are highly valued. Fatty acid-binding proteins (FABPs), which vary depending on the type of cancer, are small (14–15 kDa) proteins belonging to the lipid-binding protein superfamily. FABPs are located in many tissues and play an essential role in fatty acid metabolism, cell growth, and proliferation. It is suggested that they can be used as tumor markers. The objective of this work is to study the effects of curcumin and juglone on cell viability and to evaluate their anticancer and cytotoxic effects and changes in the FABP5 and FABP9 gene expression and protein levels in breast cancer cell lines MCF-7 and MDA-MB-231. Information on FABP5 and FABP9 gene expression in BRCA (breast invasive cancer) and normal cells was collected from the GEPIA2 and UALCAN databases. MTT analysis revealed that the IC50 (concentration of half maximal inhibitory effect) in MCF-7 cells was 22.4 and 16.3 μM for curcumin and juglone, respectively, and in MDA-MB-231 cells, 10.4 and 3.4 μM for curcumin and juglone, respectively. In both cell lines, FABP5 and FABP9 gene expression and protein levels were also analyzed. We found that treatment of MCF-7 cells with curcumin and juglone reduced cell viability, expression of the FABP5 and FABP9 genes, and the levels of the FABP5 and FABP9 proteins. In the MDA-MB-231 cell line, the FABP5 and FABP9 levels were increased at low doses of curcumin and juglone and decreased at higher doses.
Yazarlar (5)
1
D. Soyler
2
Emine N. Korucu
3
Esma Menevşe
4
A. A. Azzawri
5
Dudu Erkoc-Kaya
ORCID: 0000-0003-0114-6602
Anahtar Kelimeler
breast cancer
curcumin
FABP5
FABP9
juglone
Kurumlar
Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
1
Atıf
5
Yazar
5
Anahtar Kelime