Scopus
YÖKSİS DOI Eşleşti
SJR Q2
Effect of putrescine supplementation to in vitro maturation medium on embryo development and quality in cattle
Animal Biotechnology · Ocak 2023
YÖKSİS Kayıtları
Effect of putrescine supplementation to in vitro maturation medium on embryo development and quality in cattle
Animal Biotechnology · 2023 SCI-Expanded
Doç. Dr. HASAN ALKAN →
Effect of putrescine supplementation to in vitro maturation medium on embryo development and quality in cattle
Informa UK Limited · 2023 SCI-Expanded
Dr. Öğr. Üyesi MUSTAFA BODU →
Effect of putrescine supplementation to in vitro maturation medium on embryo development and quality in cattle
Animal Biotechnology · 2023 SCI-Expanded
Doç. Dr. KÜBRA KARAKAŞ ALKAN →
Effect of putrescine supplementation to in vitro maturation medium on embryo development and quality in cattle
Animal Biotechnology · 2023 SCI-Expanded
Doç. Dr. FATMA SATILMIŞ →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Assessment of A1 and A2 variants in the CNS2 gene of some cattle breeds by using ACRS-PCR method
2023 ISSN: 1049-5398 SCI
Doç. Dr. ÖZCAN ŞAHİN →
Association between MYLK4 gene polymorphism and growth traits at different age stages in Anatolian black cattle
2020 ISSN: 1049-5398 SCI-Expanded
Prof. Dr. İBRAHİM AYTEKİN →
Determination of the relationship between Anatolian black cattle growth properties and myostatin, GHR and Pit-1 gene
2021 ISSN: 1049-5398 SCI-Expanded Q2
Prof. Dr. UĞUR ZÜLKADİR →
Association between polymorphisms of Myf5, MSTN and CAST genes and fattening performance in Brown Swiss and Holstein cattle breeds
2021 ISSN: 1049-5398 SCI-Expanded Q2
Prof. Dr. UĞUR ZÜLKADİR →
Determination of the relationship between Anatolian black cattle growth properties and myostatin, GHR and Pit-1 gene
2022 ISSN: 1049-5398 SCI-Expanded Q2
Prof. Dr. UĞUR ZÜLKADİR →
Effect of putrescine supplementation to in vitro maturation medium on embryo development and quality in cattle
2023 ISSN: 1049-5398 SCI-Expanded Q1
Doç. Dr. HASAN ALKAN →
Effect of putrescine supplementation to in vitro maturation medium on embryo development and quality in cattle
2023 ISSN: 1049-5398 SCI-Expanded Q2
Dr. Öğr. Üyesi MUSTAFA BODU →
Effect of putrescine supplementation to in vitro maturation medium on embryo development and quality in cattle
2023 ISSN: 1049-5398 SCI-Expanded Q1
Doç. Dr. KÜBRA KARAKAŞ ALKAN →
Effect of putrescine supplementation to in vitro maturation medium on embryo development and quality in cattle
2023 ISSN: 1049-5398 SCI-Expanded Q1
Doç. Dr. FATMA SATILMIŞ →
Makale Bilgileri
Dergi
Animal Biotechnology
ISSN10495398
Yayın TarihiOcak 2023
Cilt / Sayfa34 · 3887-3896
Scopus ID2-s2.0-85165496982
Özet
This study aimed to investigate the effect of putrescine supplementation to maturation medium during in vitro embryo production in cattle on maturation and embryo development/quality. Oocytes obtained from the ovaries of Holstein cattle were used in the study. Obtained cumulus-oocyte complexes were evaluated according to morphological structure, cytoplasmic features, and cumulus cell number, and only Category-I ones were used in the study. Before the in vitro maturation step, oocytes were randomly divided into two groups. In the first group (Putrescine group, n = 159), 0.5 mM putrescine was added to the maturation medium before in vitro maturation. No addition was applied to the maturation medium of the second group (Control group, n = 149). Cumulus expansion degrees of oocytes following maturation (Grade I: poor, Grade II: partial, and Grade III: complete) were determined. In addition, the meiosis of oocytes after maturation was evaluated by differential staining. Then the oocytes were left for fertilization with sperm and finally, possible zygotes were transferred to the culture medium. After determining the developmental stages and quality of the embryos after in vitro culture, only the embryos at the blastocyst stage were stained with the differential staining method to determine the cell numbers. When the cumulus expansion degrees of the groups were evaluated, the Grade III cumulus expansion rate in the putrescine group was higher than the control group (74.21% and 60.4%; respectively) and the Grade I expansion rate (11.95% and 26.17%; respectively) was found lower (p <.05). When the resumption of meiosis was evaluated according to the cumulus expansion degrees, it was determined that the rate of resumption of meiosis increased as the cumulus expansion increased. In addition, the cleavage rates of oocytes and reaching the blastocyst in the putrescine group were found to be higher than in the control group (p <.05). Moreover, inner cell mass, trophectoderm cells, and total cell counts were found to be higher in blastocysts obtained after the putrescine supplementation to the maturation medium compared to the control group (p <.05). As a result, it was determined that the putrescine supplementation to the maturation medium during in vitro embryo production in cattle increased the degree of cumulus expansion and the rate of resumption of meiosis. In addition, putrescine supplementation was thought to increase the rate of reaching the blastocyst of oocytes due to better cell development in embryos.
Yazarlar (6)
1
Esra Bicici
2
Fatma Satilmiş
3
Mustafa Bodu
ORCID: 0000-0002-8432-7347
4
Mehmet Akif Demirel
5
Kubra Karakas Alkan
6
Hasan Alkan
ORCID: 0000-0001-8332-5334
Anahtar Kelimeler
Blastocyst development
cumulus expansion
IVEP
maturation
putrescine
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Animal Biotechnology
Q2
OA
SJR Skoru0,486
H-Index41
YayıncıTaylor and Francis Ltd.
ÜlkeUnited States
Animal Science and Zoology (Q2)
Bioengineering (Q3)
Biotechnology (Q3)
Metrikler
5
Atıf
6
Yazar
5
Anahtar Kelime