Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Protective effects of Nigella sativa oil against bisphenol A-induced testicular toxicity in rats
Medycyna Weterynaryjna · Ocak 2022
YÖKSİS Kayıtları
Protective effects of Nigella sativa oil against bisphenol A-induced testicular toxicity in rats
Medycyna Weterynaryjna - Redakcja · 2023 SCI-Expanded
DOÇENT MEHMET BURAK ATEŞ →
Protective effects of Nigella sativa oil against bisphenol A-induced testicular toxicity in rats
Medycyna Weterynaryjna · 2022 SCI-Expanded
DOÇENT DURMUŞ HATİPOĞLU →
Protective effects of Nigella sativa oil against bisphenol A-induced testicular toxicity in rats
Medycyna Weterynaryjna - Redakcja · 2023 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUSTAFA BODU →
Makale Bilgileri
DergiMedycyna Weterynaryjna
Yayın TarihiOcak 2022
Cilt / Sayfa77
Scopus ID2-s2.0-85143159861
Erişim🔓 Açık Erişim
Özet
The study investigated the protective effect of Nigella sativa (NS) oil against testicular damage induced by bisphenol A (BPA). A total of 36 Wistar Albino rats were divided into 4 groups: control (1 ml olive oil), NS (5 ml/kg NS), BPA (100 mg/kg BPA in 1 ml olive oil), BPA+NS (100 mg/kg BPA + 5 ml/kg NS). During the 30-day experiment, all substances were administered by oral gavage. The levels of testosterone and LH in serum, as well as MDA, GSH, SOD, TNF-α, IL-6 and IL-10 levels in testicular tissue were measured by ELISA. Spermatological examinations and histopathological analyses of testicular tissue were also performed. BPA was found to raise LH while reducing the serum testosterone level. BPA caused a decrease in GSH, SOD, TNF-α and IL-6, but an increase in MDA and IL-10. BPA also reduced sperm motility, plasma membrane integrity, mitochondrial activity and acrosome membrane integrity. Oedema, hyperaemia, mononuclear cell infiltration in the interstitial tissue, as well as giant cell formations, degeneration and necrosis in the seminiferous tubules were seen in the BPA group. In addition, reductions in germinative epithelium, tubule diameter and the Johnson testicular score were noted. In rats receiving NS, hormonal profile, oxidative stress markers and cytokine levels were within normal limits. NS improved sperm motility, mitochondrial activity (JC1), plasma membrane integrity (SYBR-14) and partially acrosome membrane integrity (FITC-PNA). NS reduced testis inflammation, significantly ameliorated the testicular score and, to a lesser extent, increased germinative epithelium thickness and tubule diameter. It was concluded that NS has a protective effect against BPA-induced testicular toxicity.
Yazarlar (3)
1
Durmus Hatipoglu
ORCID: 0000-0003-3790-7821
2
Mehmet Burak Ates
ORCID: 0000-0003-1297-426X
3
Mustafa Bodu
ORCID: 0000-0002-8432-7347
Anahtar Kelimeler
antioxidant
BPA
histopathology
Nigella sativa
testis
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey