Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
A comparison between two different automated total 25-hydroxyvitamin D immunoassay methods using liquid chromatography-tandem mass spectrometry
Biochemia Medica · Ocak 2015
YÖKSİS Kayıtları
A comparison between two different automated total 25 hydroxyvitamin D immunoassay methods using liquid chromatography tandem mass spectrometry
Biochemia Medica · 2015 SCI-Expanded
PROFESÖR BAHADIR ÖZTÜRK →
A comparison between two different automated total 25 hydroxyvitamin D immunoassay methods using liquid chromatography tandem mass spectrometry
Biochemia Medica · 2015 SCI-Expanded
PROFESÖR ALİ ÜNLÜ →
Makale Bilgileri
DergiBiochemia Medica
Yayın TarihiOcak 2015
Cilt / Sayfa25 · 430-438
Scopus ID2-s2.0-84943575674
Erişim🔓 Açık Erişim
Özet
Introduction: Total 25-hydroxyvitamin D [25(OH)D] is the most reliable indicator of vitamin D status. In this study, we compared two automated immunoassay methods, the Abbott Architect 25-OH Vitamin D assay and the Roche Cobas Vitamin D total assay, with the liquid chromatographytandem mass spectrometry (LC-MS/MS). Materials and methods: One hundred venous blood samples were randomly selected from routine vitamin D tests. Two of the serum aliquots were analyzed at the Abbott Architect i2000 and the Roche Cobas 6000’s module e601 in our laboratory within the same day. The other serum aliquots were analyzed at the LC-MS/MS in different laboratory. Passing-Bablok regression analysis and Bland-Altman plot were used to compare methods. Inter-rater agreement was analyzed using kappa (κ) analysis. Results: The Roche assay showed acceptable agreement with the LC-MS/MS based on Passing-Bablok analysis (intercept: -5.23 nmol/L, 95% CI: -8.73 to 0.19; slope: 0.97, 95% CI: 0.77 to 1.15). The Abbott assay showed proportional (slope: 0.77, 95% CI: 0.67 to 0.85) and constant differences (intercept: 17.08 nmol/L; 95% CI: 12.98 to 21.39). A mean bias of 15.1% was observed for the Abbott and a mean bias of -14.1% was observed for the Roche based on the Bland-Altman plots. We found strong to nearly perfect agreement in vitamin D status between the immunoassays and LC-MS/ MS. (κ: 0.83 for Abbott, κ: 0.93 for Roche) using kappa analysis. Conclusion: Both immunoassays demonstrated acceptable performance, but the Roche Cobas assay demonstrated better performance than the Abbott Architect in the studied samples.
Yazarlar (6)
1
Fatma Emel Kocak
ORCID: 0000-0001-5549-3883
2
Bahadır Öztürk
3
Ozben Ozden Isiklar
4
Ozlem Genc
5
Ali Ünlü
ORCID: 0000-0002-9991-3939
6
Irfan Altuntas
Anahtar Kelimeler
25-Hydroxyvitamin D
Chromatography
Immunoassay
Methods
Tandem mass spectrometry
Kurumlar
Dumlupinar Üniversitesi
Kutahya Turkey
Selçuk Üniversitesi
Selçuklu Turkey
T.C. Ministry of Health Kütahya Health Sciences University Evliya Çelebi Education and Research Hospital
Kutahya Turkey
Metrikler
22
Atıf
6
Yazar
5
Anahtar Kelime