Scopus
YÖKSİS Eşleşti
Evaluation of haploidization efficiency in winter squash (Cucurbita maxima Duch.) and pumpkin (cucurbita moschata Duch.) through anther culture
Plant Cell, Tissue and Organ Culture · Kasım 2016
YÖKSİS Kayıtları
Evaluation of haploidization efficiency in winter squash Cucurbita maxima Duch and pumpkin Cucurbita moschata Duch through anther culture
Plant Cell, Tissue and Organ Culture (PCTOC) · 2016 SCI-Expanded
PROFESÖR ERTAN SAİT KURTAR →
Evaluation of haploidization efficiency in winter squash Cucurbita maxima Duch and pumpkin Cucurbita moschata Duch through anther culture
Plant Cell, Tissue and Organ Culture (PCTOC) · 2016 SSCI
PROFESÖR ERTAN SAİT KURTAR →
Makale Bilgileri
DergiPlant Cell, Tissue and Organ Culture
Yayın TarihiKasım 2016
Cilt / Sayfa127 · 497-511
Scopus ID2-s2.0-84984780840
Özet
The production of large numbers of haploids is the crucial point of the dihaploidisation process. Although in vitro haploid plants were successfully produced by irradiated pollen technique in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.), the frequency is still insufficient for using in a large-scale breeding programme. Thus, the present study was conducted to determine the efficacy of the anther culture technique on the production of in vitro haploids in the aforementioned species for which there have been no successful reports concerning by androgenesis. The anthers at uninucleate microspore stage were collected at different florescence times and then cultured on a solid MS medium supplemented by different combinations of 2,4-D (2,4-dichlorophenoxyacetic acid), BAP (6-benzylaminopurine), KN (kinetin) with the constant addition of NAA (naphthalene acetic acid) to induce callogenesis, embryogenesis and plantlet initiation. The combination of PGR, genotype and anther collection time played an important role in the androgenic response. The highest response was obtained from 57Sİ21 and G9 lines with the combination of 2.0 or 4.0 mg/l BAP + 0.05 mg/l NAA (E6 medium) at the first anther collection time. Plantlets were regenerated and rooted on MS medium supplemented by 0.01 mg/l IAA. In total, 74 plants were recovered and propagated with micro-cuttings. The ploidy analyses revealed that 35 plants (47.3 %) were haploid (n = 20), and the others (52.7 %) were diploid (2n = 40).
Yazarlar (3)
1
Ertan Sait Kurtar
2
Ahmet Balkaya
3
Dilek Kandemir
Anahtar Kelimeler
Anther culture
Haploidisation
Winter squash and pumpkin
Kurumlar
Ondokuz Mayis Üniversitesi
Samsun Turkey
Metrikler
24
Atıf
3
Yazar
3
Anahtar Kelime