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Production of callus mediated gynogenic haploids in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.)

Czech Journal of Genetics and Plant Breeding · Ocak 2018

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YÖKSİS Kayıtları
Production of callus mediated gynogenic haploids in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.)
Czech Journal of Genetics and Plant Breeding · 2018 SCI-Expanded
PROFESÖR ERTAN SAİT KURTAR →
Production of callus mediated gynogenic haploids in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.)
Check Journal of Genetics and Plant Breeding · 2017 SCI-Expanded
PROFESÖR ERTAN SAİT KURTAR →

Makale Bilgileri

DergiCzech Journal of Genetics and Plant Breeding
Yayın TarihiOcak 2018
Cilt / Sayfa54 · 9-16
DOI10.17221/30/2017-CJGPB
Scopus ID2-s2.0-85044478700
Erişim🔓 Açık Erişim
Özet Although haploids were successfully produced via irradiated pollen technique and anther culture in Cucurbita maxima and Cucurbita moschata, the haploidization efficiency is still low due to genotype dependence. Thus, as an alternative technique, the efficacy of the ovule culture was investigated. Ovules were extracted at different flowering time and then cultured on a solid MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), benzylaminopurine (BAP), thidiazuron (TDZ), and naphthaleneacetic acid (NAA) to induce callogenesis and plant regeneration. The gynogenic response was influenced by the combination of plant growth regulators, genotype and culture time. The medium containing of 4.0 mg/l BAP + 0.05 mg/l NAA + 0.1 mg/l TDZ provided the highest response at anthesis time. Plantlets were rooted and elongated on a solid MS medium supplemented with 0.01 mg/l indole-3-acetic acid (IAA) + 1.0 mg/l BAP. The ploidy observations of 122 plants revealed that 70 plants were haploid, 46 plants were diploid and the others were mixoploid.

Yazarlar (3)

1
Ertan Sait Kurtar
2
Ahmet Balkaya
3
Mehtap Ozbakir Ozer

Anahtar Kelimeler

Cucurbits Dihaploidization Growth regulators Ovule culture

Kurumlar

Karadeniz Tarimsal Arastirma Enstitüsü, Samsun
Samsun Turkey
Ondokuz Mayis Üniversitesi
Samsun Turkey
Selçuk Üniversitesi
Selçuklu Turkey

Metrikler

16
Atıf
3
Yazar
4
Anahtar Kelime

Sistemimizdeki Yazarlar

EK
ERTAN SAİT KURTAR
PROFESÖR

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