Scopus
YÖKSİS Eşleşti
Molecular detection of selected tick-borne pathogens infecting cattle at the wildlife–livestock interface of Queen Elizabeth National Park in Kasese District, Uganda
Ticks and Tick-borne Diseases · Eylül 2021
YÖKSİS Kayıtları
Molecular detection of selected tick-borne pathogens infecting cattle at the wildlife\u2013livestock interface of Queen Elizabeth National Park in Kasese District, Uganda
Ticks and Tick-borne Diseases · 2021 SCI-Expanded
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Makale Bilgileri
DergiTicks and Tick-borne Diseases
Yayın TarihiEylül 2021
Cilt / Sayfa12
Scopus ID2-s2.0-85109199075
Özet
In Uganda, ticks and tick-borne diseases (TBDs) pose a big challenge to farmers. They reduce cattle productivity and cause severe economic damage. Several studies have documented the prevalence of tick-borne pathogens in cattle; however, their genetic characteristics and the role of wildlife-livestock interaction in the epidemiology of the TBDs are not well documented. This study assessed the prevalence and genetic diversity of various tick-borne pathogens (TBPs) as well as the risk factors associated with the occurrence of TBPs in blood samples of 208 randomly selected cattle from 16 farms located around Queen Elizabeth National Park (QENP) in Kasese District in western Uganda. Farming practices, disease challenges, and the level of wildlife-livestock interactions were assessed by a questionnaire survey amongst farm owners. Polymerase chain reaction (PCR) assays revealed that 62.9% (131/208) cattle samples were positive for one or more pathogens. Using specific PCR assays, we detected Theileria spp., Theileria parva, Anaplasma marginale, Anaplasma platys-like, and Babesia bigemina at 50.5%, 27.9%, 19.2%, 11.5% and 8.7%, respectively. We also confirmed the infection of samples by Theileria velifera and Theileria mutans after sequencing the Theileria spp. 18S rRNA gene. The risk factors associated with the occurrence of TBPs included communal grazing, herd size, age, and proximity to QENP. Phylogenetic analysis of the T. parva p104 gene showed a high identity to the previous isolates from Uganda and other East African countries and clustered closer to the buffalo (Syncerus caffer) isolates, suggesting a possible cross-species transmission. The sequences of A. marginale groEL and B. bigemina RAP-1a formed well-supported clades with high identities to the previous isolates identified from central and eastern Uganda. The isolates obtained from A. phagocytophilum 16S rRNA gene sequences showed relationship with A. platys-like, Anaplasma sp., uncultured Anaplasma species and A. phagocytophilum isolates from Africa, Asia, Europe, and the USA. The findings of the present study showed that TBDs are still a burden to farmers and that management practices in this area may increase the transmission of pathogens between livestock and wildlife.
Yazarlar (17)
1
Byamukama Benedicto
2
Patrick Vudriko
3
Maria Tumwebaze
4
Dickson Stuart Tayebwa
5
Joseph Byaruhanga
6
Martin Kamilo Angwe
7
Jixu Li
8
Eloiza May Galon
9
Aaron Ringo
10
Mingming Liu
11
Yongchang Li
12
Shengwei Ji
13
Mohamed Rizk
14
Paul Frank Adjou Moumouni
15
Seung Hun Lee
16
Ferda Sevinc
17
Xuenan Xuan
Anahtar Kelimeler
Cattle
Tick-borne pathogens
Wildlife–livestock interface
Kurumlar
Chungbuk National University
Cheongju South Korea
Faculty of Veterinary Medicine
Mansoura Egypt
Makerere University
Kampala Uganda
Obihiro University of Agriculture and Veterinary Medicine
Obihiro Japan
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
6
Atıf
17
Yazar
3
Anahtar Kelime