Scopus
YÖKSİS Eşleşti
Molecular detection and genetic characterization of Babesia, Theileria and Anaplasma amongst apparently healthy sheep and goats in the central region of Turkey
Ticks and Tick-borne Diseases · Şubat 2017
YÖKSİS Kayıtları
Molecular detection and genetic characterization of Babesia Theileria and Anaplasma amongst apparently healthy sheep and goats in the central region of Turkey
Ticks and Tick-borne Diseases · 2017 SCI-Expanded
DOÇENT ONUR CEYLAN →
Molecular detection and genetic characterization of Babesia, Theileria and Anaplasma amongst apparently healthy sheep and goats in the central region of Turkey
Ticks and Tick-borne Diseases · 2017 SCI-Expanded
DOÇENT ONUR CEYLAN →
Molecular detection and genetic chracterization of Babesia Theileria and Anaplasma amongst apparently healthy sheep and goats in the central region of Turkey
Ticks and Tick-borne Diseases · 1900 SCI-Expanded
DOÇENT ONUR CEYLAN →
Molecular detection and genetic characterization of Babesia Theileria and Anaplasma amongst apparently healthy sheep and goats in the central region of Turkey
Ticks and Tick-borne Diseases · 2017 SCI-Expanded
PROFESÖR FERDA SEVİNÇ →
Molecular detection and genetic characterization of Babesia Theileria and Anaplasma amongst apparently healthy sheep and goats in the central region of Turkey
Ticks and Tick-borne Diseases · 2017 SSCI
PROFESÖR MUTLU SEVİNÇ →
Molecular detection and genetic characterization of Babesia, Theileria and Anaplasma amongst apparently healthy sheep and goats in the central region of Turkey
Ticks and Tick-borne Diseases · 2017 SCI-Expanded
PROFESÖR MUTLU SEVİNÇ →
Makale Bilgileri
DergiTicks and Tick-borne Diseases
Yayın TarihiŞubat 2017
Cilt / Sayfa8 · 246-252
Scopus ID2-s2.0-85007298208
Özet
Babesia spp., Theileria spp. and Anaplasma spp. are significant tick-borne pathogens of livestock globally. In this study, we investigated the presence and distribution of Babesia ovis, Theileria ovis and Anaplasma ovis in 343 small ruminants (249 sheep and 94 goats) from 13 towns in the Central Anatolia region of Turkey using species-specific PCR assays. The PCR were conducted using the primers based on the B. ovis ssu rRNA (BoSSUrRNA), T. ovis ssu rRNA (ToSSUrRNA) and A. ovis major surface protein 4 (AoMSP4) genes, respectively. Fragments of these genes were sequenced for phylogenetic analysis. PCR results revealed that the overall infections of A. ovis, T. ovis and B. ovis were 60.0%, 35.9% and 5.2%, respectively. Co-infection of the animals with two or three pathogens was detected in 105/343 (30.6%) of the ovine samples. The results of sequence analysis indicated that AoMSP4 were conserved among the Turkish samples, with 100% sequence identity values. In contrast, the BoSSUrRNA and ToSSUrRNA gene sequences were relatively diverse with identity values of 98.54%-99.82% and 99.23%-99.81%, respectively. Phylograms were inferred based on the BoSSUrRNA, ToSSUrRNA and AoMSP4 sequences obtained in this study and those from previous studies. B. ovis isolates from Turkey were found in the same clade as the isolates from other countries in phylogenetic analysis. On the other hand, the Turkish T. ovis isolates in the present study formed a monophyletic grouping with the isolates from other countries in a phylogeny based on ToSSUrRNA sequences. Furthermore, phylogenetic analysis using AoMSP4 sequences showed the presence of three genotypes of A. ovis. This study provides important data for understanding the epidemiology of tick-borne diseases in small ruminants and the degree of genetic heterogeneities among these pathogens in Turkey. To our knowledge, this is the first study on the co-infection of Babesia, Theileria and Anaplasma in sheep and goats in Turkey.
Yazarlar (14)
1
Mo Zhou
2
Shinuo Cao
3
Ferda Sevinc
4
Mutlu Sevinc
5
Onur Ceylan
6
Sepil Ekici
7
Charoonluk Jirapattharasate
8
Paul Franck Adjou Moumouni
9
Mingming Liu
10
Guanbo Wang
11
Aiko Iguchi
12
Patrick Vudriko
13
Hiroshi Suzuki
14
Xuenan Xuan
Anahtar Kelimeler
Goat
Sheep
Species-specific PCR
Tick-borne pathogen
Turkey
Kurumlar
Chinese Academy of Agricultural Sciences
Beijing China
Obihiro University of Agriculture and Veterinary Medicine
Obihiro Japan
Selçuk Üniversitesi
Selçuklu Turkey
Veterinary Control and Research Institute
Konya Turkey
Metrikler
53
Atıf
14
Yazar
5
Anahtar Kelime