Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Babesia ovis secreted antigen-1 is a diagnostic marker during the active Babesia ovis infections in sheep
Frontiers in Cellular and Infection Microbiology · Ocak 2023
YÖKSİS Kayıtları
Babesia ovis secreted antigen-1 is a diagnostic marker during the active Babesia ovis infections in sheep
Frontiers in Cellular and Infection Microbiology · 2023 SCI-Expanded
PROFESÖR FERDA SEVİNÇ →
Babesia ovis secreted antigen-1 is a diagnostic marker during the active Babesia ovis infections in sheep
Frontiers in Cellular and Infection Microbiology · 2023 SCI-Expanded
DOÇENT ONUR CEYLAN →
Babesia ovis secreted antigen-1 is a diagnostic marker during the active Babesia ovis infections in sheep
Frontiers in Cellular and Infection Microbiology · 2023 SCI-Expanded
PROFESÖR FERDA SEVİNÇ →
Makale Bilgileri
DergiFrontiers in Cellular and Infection Microbiology
Yayın TarihiOcak 2023
Cilt / Sayfa13
Scopus ID2-s2.0-85169620037
Erişim🔓 Açık Erişim
Özet
Ovine babesiosis caused by Babesia ovis is an economically significant disease. Recently, a few B. ovis-specific proteins, including recombinant B. ovis secreted antigen-1 (rBoSA1), have been identified. Immunological analyses revealed that rBoSA1 resides within the cytoplasm of infected erythrocytes and exhibits robust antigenic properties for detecting anti-B. ovis antibodies. This protein is released into the bloodstream during the parasite’s development. It would be possible to diagnose active infections by detecting this secretory protein. For this purpose, a rBoSA1-specific polyclonal antibody-based sandwich ELISA was optimized in this study. Blood samples taken from the naturally (n: 100) and experimentally (n: 15) infected sheep were analyzed for the presence of native BoSA1. The results showed that native BoSA1 was detectable in 98% of naturally infected animals. There was a positive correlation between parasitemia level in microscopy and protein density in sandwich ELISA. Experimentally infected animals showed positive reactions from the first or second day of inoculations. However, experimental infections carried out by Rhipicephalus bursa ticks revealed the native BoSA1 was detectable from the 7th day of tick attachment when the parasite began to be seen microscopically. Sandwich ELISA was sensitive enough to detect rBoSA1 protein at a 1.52 ng/ml concentration. Additionally, no serological cross-reactivity was observed between animals infected with various piroplasm species, including Babesia bovis, B. bigemina, B. caballi, B. canis, B. gibsoni, Theileria equi, and T. annulata. Taken collectively, the findings show that the rBoSA1-specific polyclonal antibody-based sandwich ELISA can be successfully used to diagnose clinical B. ovis infections in sheep at the early stage.
Yazarlar (8)
1
Ferda Sevinc
2
Mo Zhou
3
Shinuo Cao
4
Onur Ceylan
5
Mehmet Can Ulucesme
ORCID: 0000-0002-4492-143X
6
Sezayi Ozubek
7
Munir Aktas
ORCID: 0000-0002-3188-8757
8
Xuenan Xuan
Anahtar Kelimeler
BoSA1
cross-reaction
diagnosis
parasitemia
sandwich ELISA
Kurumlar
Firat Üniversitesi
Elazig Turkey
Jiangsu Agri-animal Husbandry Vocational College
Taizhou China
Obihiro University of Agriculture and Veterinary Medicine
Obihiro Japan
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
2
Atıf
8
Yazar
5
Anahtar Kelime