Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Tarantula cubensis alcohol extract enhances the tumoricidal effect of capecitabine via multiple pathways in azoxymethane-induced colorectal cancer in rats
Tropical Journal of Pharmaceutical Research · Şubat 2024
YÖKSİS Kayıtları
Tarantula cubensis alcoholic extract enhances the tumoricidal effect of capecitabine via multiple pathways in azoxymethane-induced colorectal cancer in rats
Tropical Journal of Pharmaceutical Research · 2024 SCI-Expanded
PROFESÖR ÖZGÜR ÖZDEMİR →
Tarantula cubensis alcoholic extract enhances the tumoricidal effect of capecitabine via multiple pathways in azoxymethane-induced colorectal cancer in rats
Tropical Journal of Pharmaceutical Research · 2024 SCI-Expanded
PROFESÖR MEHMET TUZCU →
Tarantula cubensis alcoholic extract enhances the tumoricidal effect of capecitabine via multiple pathways in azoxymethane-induced colorectal cancer in rats
Tropical Journal of Pharmaceutical Research · 2024 Scopus
ARAŞTIRMA GÖREVLİSİ ZEYNEP ÇELİK KENAR →
Makale Bilgileri
DergiTropical Journal of Pharmaceutical Research
Yayın TarihiŞubat 2024
Cilt / Sayfa23 · 291-297
Scopus ID2-s2.0-85188745206
Erişim🔓 Açık Erişim
Özet
Purpose: To evaluate the effect of a combination of Tarantula cubensis alcohol extract (TCAE) and capecitabine (CAP) in the treatment of azoxymethane (AOM)-induced colorectal cancer (CRC). Methods: Forty-two Wistar albino rats were divided into 7 groups with 6 rats in each group. The groups consisted of Control (C), Control+TCAE (C-TCAE), Control+CAP (C-CAP), Cancer control (CC), Cancer+TCAE (CC-TCAE), Cancer+CAP (CC-CAP) and Cancer+CAP+TCAE (CC-CAP+TCAE). To induce CRC, AOM (15 mg/kg) was administered to rats subcutaneously (sc) twice at a one-week interval to all the groups except control. From the 15th week, TCAE (0.2 mL/rat sc) was administered to CC-TCAE group every 3 days for 4 weeks, and CAP (40 mg/kg/day) was administered by gavage to CC-CAP group for 4 weeks. In CC-CAP+TCAE group, TCAE (0.2 mL/rat sc) was administered every 3 days for 4 weeks, and CAP (40 mg/kg/day) was administered gavage for 4 weeks. Animals were treated for 18 weeks. Aberrant crypt foci (ACF) were evaluated histopathologically among CC, CC-TCAE, CC-CAP, and CC-CAP+TCAE groups. β-catenin, CD15, Proliferating Cell Nuclear Antigen (PCNA), and Nuclear Factor kappa B (NF-κB) expression levels were immunohistochemically compared among all groups. Results: Histopathologically, ACF scores were significantly increased in CC group, while a significant decrease in the relevant scores (p < 0.001) was observed in CC-CAP and CC-CAP+TCAE treatment groups, and the lowest scores were in CC-CAP+TCAE group. Immunohistochemically, in CC group, β-catenin, Nuclear Factor kappa B (NF-κB), Proliferating Cell Nuclear Antigen (PCNA) and CD15 expressions were highly irregular. CC-CAP and CC-CAP+TCAE groups had significantly reduced expressions (p < 0.001), and the lowest expressions were in CC-CAP+TCAE group. Conclusion: The combined use of TCAE and CAP in treatment of CRC has a synergistic effect and increases the anticancer efficacy of TCAE, and CAP. More studies at the molecular level are needed in the future to demonstrate the clinical benefit of TCAE supplementation during the treatment of CRC with CAP.
Yazarlar (6)
1
G. Akcakavak
2
Zeynep Celik
ORCID: 0000-0002-9667-5728
3
Ozhan Karatas
4
Osman Dogan
5
O. Ozdemir
6
Mehmet Tuzcu
Anahtar Kelimeler
Azoxymethane
CD15
Colorectal cancer
NF-κB
PCNA
β-catenin
Kurumlar
Aksaray Üniversitesi
Aksaray Turkey
Cumhuriyet Üniversitesi
Sivas Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
2
Atıf
6
Yazar
6
Anahtar Kelime