Scopus
🔓 Açık Erişim YÖKSİS DOI Eşleşti
SJR Q2
Real-Time Analysis of Antiproliferative Effects of Mouthwashes Containing Alcohol, Sodium Fluoride, Cetylpyridinium Chloride, and Chlorhexidine in Vitro
Biomed Research International · Ocak 2021
YÖKSİS Kayıtları
Real-Time Analysis of Antiproliferative Effects of Mouthwashes Containing Alcohol, Sodium Fluoride, Cetylpyridinium Chloride, and Chlorhexidine In Vitro
BioMed Research International · 2021 SCI-Expanded
Prof. Dr. HAYRİYE ESRA ÜLKER →
Real-Time Analysis of Antiproliferative Effects of Mouthwashes Containing Alcohol, Sodium Fluoride, Cetylpyridinium Chloride, and Chlorhexidine In Vitro
BioMed Research International · 2021 SCI-Expanded
Doç. Dr. FİRDEVS KAHVECİOĞLU →
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A Comparative Study for the Evaluation of Two Doses of Ellagic Acid on Hepatic Drug Metabolizing and Antioxidant Enzymes in the Rat
2013 ISSN: 2314-6133 SCI-Expanded 12 atıf
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Intrapulpal Thermal Changes during Setting Reaction of Glass Carbomer Using Thermocure Lamp
2016 ISSN: 2314-6133 SCI-Expanded
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Intrapulpal Thermal Changes during Setting Reaction of Glass Carbomer® Using Thermocure Lamp
2016 ISSN: 2314-6133 SCI-Expanded Q2
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2013 ISSN: 2314-6133 SCI-Expanded
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2020 ISSN: 2314-6133 SCI-Expanded
Prof. Dr. NECLA DEMİR →
Real-Time Analysis of Antiproliferative Effects of Mouthwashes Containing Alcohol, Sodium Fluoride, Cetylpyridinium Chloride, and Chlorhexidine In Vitro
2021 ISSN: 2314-6133 SCI-Expanded Q2
Doç. Dr. FİRDEVS KAHVECİOĞLU →
Real-Time Analysis of Antiproliferative Effects of Mouthwashes Containing Alcohol, Sodium Fluoride, Cetylpyridinium Chloride, and Chlorhexidine In Vitro
2021 ISSN: 2314-6133 SCI-Expanded Q2
Prof. Dr. HAYRİYE ESRA ÜLKER →
Strontium Chloride: Can It Be a New Treatment Option for Ulcerative Colitis?
2014 ISSN: 2314-6133 SCI-Expanded
Prof. Dr. MEHMET TUZCU →
Bonding Effectiveness of Saliva-Contaminated Monolithic Zirconia Ceramics Using Different Decontamination Protocols
2024 ISSN: 2314-6133 ESCI
Prof. Dr. NECLA DEMİR →
Makale Bilgileri
ISSN23146133
Yayın TarihiOcak 2021
Cilt / Sayfa2021
Scopus ID2-s2.0-85118386079
Erişim🔓 Açık Erişim
Özet
Objectives. In this study, the cytotoxic responses of six different over-the-counter mouthwashes on L929 cells were analyzed by two different techniques: the traditional colorimetric tetrazolium-based reduction assay (MTT) and the modern impedance-based real-time cell analysis (RTCA) system to investigate their biocompatibility in vitro. Thus, the investigation of the antiproliferative effects of the specified materials via different techniques is vital to reach this goal. Materials and Methods. First, L929 mouse fibroblasts were exposed to the dilutions of mouthwashes for 2 minutes. After incubation, the tetrazolium reduction method was used to assess the metabolic viability of cells measured by colorimetric MTT assay and morphological inspection of cells was performed via phase-contrast microscopy. Furthermore, the effect of each mouthwash on the proliferation, morphology, and adhesion of L929 cells was monitored continuously by a noninvasive and label-free RTCA system for 140 h. Results. Our data showed that all of the mouthwashes had varying cytotoxic effects on fibroblasts compared to the control group in MTT assay. In addition to that, RTCA technology has provided the growth kinetic profiles that can be used to analyze if the treatment is causing antimitotic or DNA-damaging effect on cells. Thus, analysis via this system can tell us the mechanism of toxicity behind the cell growth inhibition in vitro. Here, we found that only mouthwash 1 moderately maintained the viability of the L929 cells, yet displaying antimitotic effects and the other mouthwashes (mouthwash 2-mouthwash 6) showed toxicity via DNA-damaging effects. Conclusions. Of the six types of mouthwash tested, the most biocompatible result was obtained from a mouthwash containing alcohol (i.e., mouthwash 1). On the other hand, sodium fluoride- (NaF-) and cetylpyridinium chloride- (CPC-) containing mouthwash (i.e., mouthwash 2) showed the most cytotoxic effect.
Yazarlar (5)
1
Mustafa Ulker
2
A. C.Tutku Çelik
3
Emine Yavuz
4
Firdevs Kahvecioğlu
ORCID: 0000-0003-4216-0849
5
H. E. Ülker
Kurumlar
Medicana Hospital
Konya Turkey
Private Celik Oral and Health Clinic
Istanbul Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
BioMed Research International
Q2
OA
SJR Skoru0,748
H-Index219
YayıncıJohn Wiley and Sons Ltd
ÜlkeUnited Kingdom
Biochemistry, Genetics and Molecular Biology (miscellaneous) (Q2)
Immunology and Microbiology (miscellaneous) (Q2)
Medicine (miscellaneous) (Q2)
Metrikler
14
Atıf
5
Yazar