Scopus
🔓 Açık Erişim YÖKSİS DOI Eşleşti
SJR Q3
Cytotoxic evaluation and LC-MS/MS analysis of aerial parts of Eryngium kotschyi Boiss. grown in Turkey
Brazilian Journal of Pharmaceutical Sciences · Ocak 2022
YÖKSİS Kayıtları
Cytotoxic evaluation and LC-MS/MS analysis of aerial parts of Eryngium kotschyi Boiss. grown in Turkey
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES · 2022 SCI-Expanded
Prof. Dr. OSMAN TUGAY →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Cytotoxic evaluation and LC-MS/MS analysis of aerial parts of Eryngium kotschyi Boiss. grown in Turkey
2022 ISSN: 1984-8250 SCI-Expanded
Prof. Dr. OSMAN TUGAY →
Makale Bilgileri
ISSN19848250
Yayın TarihiOcak 2022
Cilt / Sayfa58
Scopus ID2-s2.0-85135085960
Erişim🔓 Açık Erişim
Özet
Increasing biological activity and phytochemical investigations on Eryngium species showed its potential as pharmaceutical approach. Eryngium kotschyi Boiss. is one of the species of Eryngium genus and is endemic to Turkey. It is known that this plant is traditionally used in the South-western part of Turkey for the treatment of various diseases. This study focuses on cytotoxic activities of methanol extract and ethyl acetate, n-butanol and water sub-extracts from E. kotschyi in A549, COLO 205 and MDA-MB-231 cell lines by Sulforhodamin B assay and qualitative and quantitative determination of phytochemical constituents in active extract by LC-MS/MS. From the result of the study, it was seen that E. kotschyi ethyl acetate (EKE) sub-extract showed the strongest cytotoxic effect with the low IC50 values (50.00; 31.96 and 22.26 μg/mL in A549; COLO 205 and MDA-MB-231 cells at 48 h, respectively). Preliminary examination of the mass spectrums revealed the presence of 15 phytochemical compounds in active sub-extract and 7 of them was quantified. According to quantitative analyses the main compounds of EKE sub-extract were rosmarinic acid (485.603 µg/mgextract), chlorogenic acid (62.355 µg/mgextract) and caffeic acid (59.266 µg/mgextract). Moreover, this preliminary study on inhibitory activity of EKE sub-extract suggests further toxicologic investigations and detailed investigation on cytotoxic effect of various combinations of determined compounds.
Yazarlar (3)
1
Ayşe Kübra Karaboğa Arslan
2
Leyla Paşayeva
ORCID: 0000-0003-3860-7222
3
Osman Tugay
Anahtar Kelimeler
A549
COLO 205
Eryngium kotschyi
LC-MS/MS
MDA-MB-231
SRB
Kurumlar
Erciyes Üniversitesi
Kayseri Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Brazilian Journal of Pharmaceutical Sciences
Q3
OA
SJR Skoru0,242
H-Index53
YayıncıFaculdade de Ciencias Farmaceuticas (Biblioteca)
ÜlkeBrazil
Pharmacology, Toxicology and Pharmaceutics (miscellaneous) (Q3)
Metrikler
3
Atıf
3
Yazar
6
Anahtar Kelime