Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Evaluation of the Chemical Composition, Antioxidant and Antidiabetic Activity of Rhaponticoides iconiensis Flowers: Effects on Key Enzymes Linked to Type 2 Diabetes In Vitro, In Silico and on Alloxan-Induced Diabetic Rats In Vivo
Antioxidants · Kasım 2022
YÖKSİS Kayıtları
Evaluation of the Chemical Composition, Antioxidant and Antidiabetic Activity of Rhaponticoides iconiensis Flowers: Effects on Key Enzymes Linked to Type 2 Diabetes In Vitro, In Silico and on Alloxan-Induced Diabetic Rats In Vivo
ANTIOXIDANTS · 2024 SCI-Expanded
PROFESÖR OSMAN TUGAY →
Evaluation of the Chemical Composition, Antioxidant and Antidiabetic Activity of Rhaponticoides iconiensis Flowers: Effects on Key Enzymes Linked to Type 2 Diabetes In Vitro, In Silico and on Alloxan-Induced Diabetic Rats In Vivo
ANTIOXIDANTS · 2022 SCI-Expanded
PROFESÖR OSMAN TUGAY →
Makale Bilgileri
DergiAntioxidants
Yayın TarihiKasım 2022
Cilt / Sayfa11
Scopus ID2-s2.0-85145298520
Erişim🔓 Açık Erişim
Özet
Diabetes mellitus (DM) is one of the globally worst killer diseases. In this study, the in vitro and in vivo antidiabetic activity and antioxidant capacity were determined and the phytochemical analyses were carried out on flower extract and sub-extracts of Rhaponticoides iconiensis. The in vitro antidiabetic activity was tested with α-amylase and α-glucosidase enzyme inhibition methods and an in vivo OGTT test in healthy and alloxan-induced rats. Although, the antioxidant activity was investigated with DPPH●, ABTS●+ and FRAP tests, the phytochemical composition analysis was carried out by LC-MS/MS. The highest α-glucosidase and α-amylase activity even from positive control acarbose were found in the ethyl acetate sub-extract of R. iconiensis (IC50 = 11.737 ± 0.823 µg/mL and 84.247 ± 0.721 µg/mL, respectively). This sub-extract also was active according to the results of in vivo tests. Moreover, the highest antioxidant activity on DPPH● (IC50 = 0.126 ± 0.002 mg/mL), FRAP (at a concentration of 1 mg/mL equivalent to 3112.052 ± 2.023 mmol Fe2+) and ABTS+● (at a concentration of 0.5 mg/mL equivalent to 0.608 ± 0.005 µM Trolox) tests. In addition, LC-MS/MS analyses of the active sub-extract revealed mainly the presence of patuletin, patuletin 3,7-diglucoside, naringin and 3,4-dicaffeoylquinic acid detected in the active sub-extract. In silico molecular docking and dynamics simulations studies were performed on these compounds with α-amylase and α-glucosidase enzymes for protein–ligand interactions and stability.
Yazarlar (7)
1
Leyla Paşayeva
ORCID: 0000-0003-3860-7222
2
Hanifa Fatullayev
3
Ismail Celik
ORCID: 0000-0002-8146-1663
4
Gokhan Unal
5
Nuh Mehmet Bozkurt
ORCID: 0000-0003-2965-6719
6
Osman Tugay
7
Magda H. Abdellattif
Anahtar Kelimeler
antioxidant
diabetes
LC-MS/MS
molecular docking
molecular dynamics
Rhaponticoides iconiensis
α-amylase
α-glucosidase
Kurumlar
Erciyes Üniversitesi
Kayseri Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Taif University
Taif Saudi Arabia
Metrikler
12
Atıf
7
Yazar
8
Anahtar Kelime