Scopus
YÖKSİS DOI Eşleşti
SJR Q2
Universal cell capture by immobilized antimicrobial peptide plantaricin
Biochemical Engineering Journal · Eylül 2015
YÖKSİS Kayıtları
Universal cell capture by immobilized antimicrobial peptide plantaricin
Biochemical Engineering Journal · 2015 SCI-Expanded 1 atıf
Prof. Dr. İLKAY HİLAL GÜBBÜK →
Universal cell capture by immobilized antimicrobial peptide plantaricin
Biochemical Engineering Journal · 2015 SCI-Expanded 1 atıf
Prof. Dr. İLKAY HİLAL GÜBBÜK →
Universal cell capture by immobilized antimicrobial peptide plantaricin
Biochemical Engineering Journal · 2015 SSCI
Prof. Dr. SEMAHAT KÜÇÜKKOLBAŞI →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Universal cell capture by immobilized antimicrobial peptide plantaricin
2015 ISSN: 1369703X SSCI
Prof. Dr. SEMAHAT KÜÇÜKKOLBAŞI →
Universal cell capture by immobilized antimicrobial peptide plantaricin
2015 ISSN: 1369703X SCI-Expanded 1 atıf
Prof. Dr. İLKAY HİLAL GÜBBÜK →
Fabrication of lipase-immobilized proline-conjugated zirconium-based metal-organic framework (UiO-66-NH2) to enhance catalytic activity, stability, and enantioselectivity
2024 ISSN: 1369-703X SCI-Expanded Q2
Prof. Dr. MUSTAFA YILMAZ →
Fabrication of lipase-immobilized proline-conjugated zirconium-based metal-organic framework (UiO-66-NH2) to enhance catalytic activity, stability, and enantioselectivity
2024 ISSN: 1369-703X SCI-Expanded Q2
Prof. Dr. ELİF ÖZYILMAZ →
A novel conductometric creatinine biosensor based on solid state contact ammonium sensitive PVC NH2 membrane
2012 ISSN: 1369703X SCI-Expanded
Dr. Öğr. Üyesi VİLDAN ERCİ →
Makale Bilgileri
ISSN1369703X
Yayın TarihiEylül 2015
Cilt / Sayfa101 · 18-22
Scopus ID2-s2.0-84929485504
Özet
Plantaricin-423 is a short antimicrobial peptide and displays bactericidal activities against several foodborne pathogens and spoilage Gram positive bacteria. The goal of this study was to investigate the potential of using immobilized plantaricin for capturing microorganisms on glass arrays. The peptide used for immobilization consists of N-terminal domain of Plantaricin-423 with an additional cysteine (Pln-17C) to form disulfide bonds with thiol groups present on silanized slides. Our results showed that Pln-17C is able to capture all six strains that were tested with varying affinities. The cell capture occurred within five minutes of incubation and the binding level was highest for Listeria innocua followed by other Gram positive strains tested. Pln-17C was also able to capture Escherichia coli with lower affinity, but the binding was significantly lower for Mycobacterium smegmatis compared to other strains. In addition, we have observed that immobilized Pln-17C maintained its anti-listerial activity; however, it did not kill E. coli as expected. Our results demonstrate the feasibility of utilizing antimicrobial peptides in biosensors for pathogen detection and for creating antimicrobial surfaces. Moreover, in combination with other peptides, different target species from food-borne pathogens to biodefense agents can be captured on more stable, economic, and robust platforms.
Yazarlar (4)
1
Saadet Albayrak Guralp
2
Ilkay Hilal Gubbuk
3
Semahat Kucukkolbasi
4
Erdogan Gulari
Anahtar Kelimeler
Antimicrobial peptide
Biomedical
Biosensors
Cell capture
Immobilization
Viability
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
University of Michigan, Ann Arbor
Ann Arbor United States
Scimago Dergi (ISSN Eşleşmesi)
Biochemical Engineering Journal
Q2
SJR Skoru0,754
H-Index155
YayıncıElsevier B.V.
ÜlkeNetherlands
Bioengineering (Q2)
Biomedical Engineering (Q2)
Biotechnology (Q2)
Environmental Engineering (Q2)
Metrikler
5
Atıf
4
Yazar
6
Anahtar Kelime