Scopus
🔓 Açık Erişim
Evaluation of four different HPLC devices for hemoglobinopathy screening
Turkish Journal of Biochemistry · Temmuz 2020
Makale Bilgileri
DergiTurkish Journal of Biochemistry
Yayın TarihiTemmuz 2020
Cilt / Sayfa46 · 37-42
Scopus ID2-s2.0-85103463633
Erişim🔓 Açık Erişim
Özet
Objective: Hemoglobinopathies are a common public health problem in Turkey. In the screening of these disorders in population, cation-exchange high performance liquid chromatography (HPLC) is accepted as the gold standard method. In this study, the aim was to assess four different HPLC devices used in hemoglobinopathy screening. Materials and methods: A total of 58 blood samples were analyzed with four different HPLC methods (Bio-Rad variant II, Agilent 1100, Tosoh G8 and Trinity Ultra2 trademarks). Results: The comparison study demonstrated a good correlation between the results of each HPLC analyzer and the reference value obtained by averaging all the HbA2 results belonging to the methods tested in the study [ (Tosoh G8 (r=0.988), Bio-Rad variant II (r=0.993), Agilent 1100 (r=0.98) and Trinity Ultra2 (r=0.992) ]. HbA2 determination in the presence of HbE was interfered in both Bio- Rad variant II and Tosoh G8. Conclusion: The analyzers were found to have compatible HbA2 results but with accompanying different degrees of proportional and systematic biases. HPLC analyzers may be affected by different hemoglobin variants at different HbA2 concentrations, which is an important point to take into consideration during the evaluation of HbA2 results in thalassemia screening.
Yazarlar (8)
1
Müjgan Ercan Karadaǧ
2
Emiş Deniz Akbulut
3
Esra Firat Oǧuz
4
Fatma Meriç Yilmaz
5
Esin Avci
6
Saadet Kader
7
Gulsum Abusoglu
8
Muhittin Serdar
Anahtar Kelimeler
HbA2
Hemoglobinopathy
HPLC
Screening
Thalassemia
Kurumlar
Acıbadem Mehmet Ali Aydınlar Üniversitesi
Istanbul Turkey
Ankara City Hospital
Ankara Turkey
Bilecik Public Health Laboratory
Bilecik Turkey
Harran University, Faculty of Medicine
Sanliurfa Turkey
Pamukkale Üniversitesi Tip Fakültesi
Denizli Turkey
Vocational School of Health Services
Konya Turkey
Metrikler
3
Atıf
8
Yazar
5
Anahtar Kelime