Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Measurement of kynurenine pathway metabolites by tandem mass spectrometry
Journal of Mass Spectrometry and Advances in the Clinical Lab · Nisan 2023
YÖKSİS Kayıtları
Measurement of kynurenine pathway metabolites by tandem mass spectrometry
Elsevier BV · 2023 SCI-Expanded
PROFESÖR ALİ ÜNLÜ →
Measurement of kynurenine pathway metabolites by tandem mass spectrometry
Elsevier BV · 2023 SCI-Expanded
PROFESÖR ALİ ÜNLÜ →
Measurement of kynurenine pathway metabolites by tandem mass spectrometry
Elsevier BV · 2023 SCI-Expanded
PROFESÖR SEDAT ABUŞOĞLU →
Measurement of kynurenine pathway metabolites by tandem mass spectrometry
Elsevier BV · 2023 SCI-Expanded
PROFESÖR ALİ ÜNLÜ →
Measurement of kynurenine pathway metabolites by tandem mass spectrometry
Elsevier BV · 2023 SCI-Expanded
PROFESÖR FATMA HÜMEYRA YERLİKAYA AYDEMİR →
Measurement of kynurenine pathway metabolites by tandem mass spectrometry
Elsevier BV · 2023 SCI-Expanded
PROFESÖR ALİ ÜNLÜ →
Measurement of kynurenine pathway metabolites by tandem mass spectrometry
Elsevier BV · 2023 SCI-Expanded
DOÇENT GÜLSÜM ABUŞOĞLU →
Makale Bilgileri
DergiJournal of Mass Spectrometry and Advances in the Clinical Lab
Yayın TarihiNisan 2023
Cilt / Sayfa28 · 114-121
Scopus ID2-s2.0-85152726789
Erişim🔓 Açık Erişim
Özet
Objectives: Recent studies have shown that derangements in kynurenine pathway metabolite levels are associated with various pathologies such as neurodegenerative diseases, schizophrenia, depression, bipolar disorder, rheumatoid arthritis, and cancer. Therefore, reliable, accurate, fast, and multiplex measurement methods for kynurenines have become increasingly important. This study aimed to validate a new mass spectrometric method for analyzing tryptophan metabolites. Methods: A tandem mass spectrometric method, including protein precipitation and evaporation steps, was developed to measure serum levels of tryptophan, kynurenine, kynurenic acid, 3-hydroxykynurenine, and 3-hydroxyanthranilic acid. Samples were separated using a Phenomenex Luna C18 reversed-phase column. The kynurenine pathway metabolites were detected by tandem mass spectrometry. The developed method was validated according to Clinical & Laboratory Standards Institute (CLSI) guidelines and applied to hemodialysis samples. Results: The developed method was linear at the concentrations of 48.8 – 25,000, 0.98 – 500, 1.2–5000, 1.2–5000, and 0.98–250 ng/mL for tryptophan, kynurenic acid, kynurenine, 3-hydroxyanthranilic acid, and 3-hydroxykynurenine, respectively. The imprecisions were less than 12 %. The median serum concentrations of tryptophan, kynurenine, kynurenic acid, 3-hydroxykynurenine, and 3-hydroxyanthranilic acid were 10530, 1100, 218, 17.6, and 25.4 ng/mL in pre-dialysis blood samples, respectively. They were 4560, 664, 135, 7.4, and 12.8 ng/mL in post-dialysis blood samples, respectively. Conclusions: A fast, simple, cost-effective, accurate, robust, and validated tandem mass spectrometric method was developed, and the method was successfully used for the quantitation of kynurenine pathway metabolite concentrations in hemodialysis patients.
Yazarlar (5)
1
Sedat Abusoglu
ORCID: 0000-0002-2984-0527
2
Duygu Eryavuz Onmaz
ORCID: 0000-0001-8564-1824
3
Gulsum Abusoglu
4
Fatma Humeyra Yerlikaya
5
Ali Ünlü
ORCID: 0000-0002-9991-3939
Anahtar Kelimeler
Kynurenine pathway
Mass spectrometry
Tryptophan
Validation
Kurumlar
Selçuk Tip Fakültesi
Konya Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
5
Atıf
5
Yazar
4
Anahtar Kelime