Scopus
YÖKSİS Eşleşti
Investigation the effects of bee venom and H-dental-derived mesenchymal stem cells on non-small cell lung cancer cells (A549)
Molecular Biology Reports · Aralık 2024
YÖKSİS Kayıtları
Investigation the effects of bee venom and H-dental-derived mesenchymal stem cells on non-small cell lung cancer cells (A549)
MOLECULAR BIOLOGY REPORTS · 2024 SCI-Expanded
PROFESÖR HÜSAMETTİN VATANSEV →
Investigation the effects of bee venom and H-dental-derived mesenchymal stem cells on non-small cell lung cancer cells (A549)
Springer Science and Business Media LLC · 2024 SCI-Expanded
PROFESÖR BAHADIR ÖZTÜRK →
Investigation the effects of bee venom and H-dental-derived mesenchymal stem cells on non-small cell lung cancer cells (A549)
MOLECULAR BIOLOGY REPORTS · 2024 SCI-Expanded
PROFESÖR HÜSAMETTİN VATANSEV →
Makale Bilgileri
DergiMolecular Biology Reports
Yayın TarihiAralık 2024
Cilt / Sayfa51
Scopus ID2-s2.0-85178893188
Özet
Background: Lung cancer, one of the most common oncological diseases worldwide, continues to be the leading cause of cancer-related deaths. The development of new approaches for lung cancer, which still has a low survival rate despite medical advances, is of great importance. Methods and results: In this study, bee venom (BV), conditioned medium of MSCs isolated from dental follicles (MSC-CM) and cisplatin were applied at different doses and their effects on A549 cell line were evaluated. Dental follicles were used as a source of MSCs source and differentiation kits, and characterization studies (flow cytometry) were performed. Cell viability was measured by the MTT method and apoptosis was measured by an Annexin V-FITC/PI kit on flow cytometer. IC50 dose values were determined according to the 24th hour and were determined as 15.8 µg/mL for BV, 10.78% for MSC-CM and 5.77 µg/mL for cisplatin. IC50 values found for BV and MSC-CM were also given in combination and the effects were observed. It was found that the applied substances caused BV to decrease in cell viability and induced apoptosis in cells. In addition to the induction of apoptosis in BV, MSC-CM, and combined use, all three applications led to an increase in Bax protein expression and a decrease in Bcl-2 protein expression. The molecular mechanism of anticancer activity through inhibition of Bax and Bcl-2 proteins and the NF-κB signaling pathway may be suggested. Conclusion: Isolated MSCs in our study showed anticancer activity and BV and MSC-CM showed synergistic antiproliferative and apoptotic effects.
Yazarlar (3)
1
Fatma Sengul
ORCID: 0000-0002-7097-6266
2
Hüsamettin Vatansev
3
Bahadır Öztürk
Anahtar Kelimeler
A549
Apoptosis
Bee venom
Mesenchymal stem cells
Western blot
Kurumlar
Selçuk Tip Fakültesi
Konya Turkey
University of Adiyaman
Adiyaman Turkey
Metrikler
1
Atıf
3
Yazar
5
Anahtar Kelime