Scopus
Protective effect of Petroselinum crispum methanolic extract against acrylamide-induced reproductive toxicity in male rats through NF-ĸB, kinesin, steroidogenesis pathways
Reproductive Toxicology · Haziran 2024
Makale Bilgileri
DergiReproductive Toxicology
Yayın TarihiHaziran 2024
Cilt / Sayfa126
Scopus ID2-s2.0-85190899830
Özet
This study examined the protective effects of a Petroselinum crispum (P. crispum) methanolic extract on reproductive dysfunction induced by acrylamide in male rats. A total of 40 rats were divided into four groups (n=10). The control group received distilled water, the acrylamide group received 10 mg/kg of acrylamide, the P. crispum group received 100 mg/kg of P. crispum extract, and the combined group was pretreated with P. crispum for two weeks before co-administration of P. crispum and acrylamide. All administrations were administered orally using a gastric tube for eight weeks. Acrylamide decreased testosterone levels but did not affect levels of FSH or LH. It also increased testicular levels of (MDA) malondialdehyde and reduced activity of (SOD) superoxide dismutase and impairment of sperm parameters. Furthermore, the administration of acrylamide resulted in an elevation of tumor necrosis factor-alpha (TNF-α) levels and a reduction in the levels of steroidogenic acute regulatory protein (STAR) and cytochrome P450scc (P450scc). Acrylamide negatively affected the histopathological outcomes, Johnsen's score, the diameter of seminiferous tubules, and the thickness of the germinal epithelium. It also upregulated the expression of NF-ĸB P65 and downregulated the expression of kinesin motor protein. In contrast, treatment with P. crispum extract restored the levels of antioxidant enzymes, improved sperm parameters, and normalized the gene expression of TNF-α, IL-10, IL-6, iNOS, NF-ĸB, STAR, CYP17A1, 17β-HSD and P450scc. It also recovered testicular histological parameters and immunoexpression of NF-ĸB P65 and kinesin altered by acrylamide. P. crispum showed protective effects against acrylamide-induced reproductive toxicity by suppressing oxidative damage and inflammatory pathways.
Yazarlar (17)
1
Ahmed M.E. Shipa
2
Khaled A. Kahilo
3
Samir A. Elshazly
4
Ehab S. Taher
5
Nasr E. Nasr
6
Badriyah S. Alotaibi
7
Essam A. Almadaly
8
Mona Assas
9
Walied Abdo
10
Tarek K. Abouzed
11
Abdulati Elsanusi Salem
12
Damla Kirci
13
Hesham R. El-Seedi
14
Mohamed S. Refaey
15
Nermin I. Rizk
16
Mustafa Shukry
17
Doaa A. Dorghamm
Anahtar Kelimeler
Acrylamide
Kinesin
Male fertility
Petroselinum crispum
Rats
Reproductive toxicity
Testis
Kurumlar
Biochemistry Department University of Misrata
Libya
Faculty of Fisheries and Aquaculture Sciences
Kafr el-Sheikh Egypt
Faculty of Pharmacy
Sadat City Egypt
Faculty of Veteinary Medicine
Kafr el-Sheikh Egypt
Islamic University of Madinah
Medina Saudi Arabia
Menoufia University Faculty of Medicine
Shibin El Kom Egypt
Princess Nourah Bint Abdulrahman University
Riyadh Saudi Arabia
Selçuk Üniversitesi
Selçuklu Turkey
Zarqa University
Zarqa Jordan