Scopus
Combination antioxidant effect of α-tocoferol and erdosteine in ischemia-reperfusion injury in rat model
International Urology and Nephrology · Eylül 2010
Makale Bilgileri
DergiInternational Urology and Nephrology
Yayın TarihiEylül 2010
Cilt / Sayfa42 · 647-655
Scopus ID2-s2.0-78549275822
Özet
Introduction: Renal ischemia/reperfusion (I/R) which is an important cause of renal dysfunction is inevitable in renal transplantation, surgical revascularization of the renal artery, partial nephrectomy and treatment of suprarenal aortic aneurysms. Aim: The purpose of this study was to investigate the efficacy of α-tocopherol and erdosteine combination in the reduction in injury induced by ROS in a rat model of renal ischemia-reperfusion. Materials and methods: Thirty-six- male Wistar albino rats weighing 200-250 g were utilized for this study. Rats were divided into six groups, and each group was consistent of six rats: (1) sham-operated (control), (2) ischemia group (3) I/R group, (4) I/R/α-tocoferol group (5) I/erdosteine group (6). I/R/α-tocoferol and erdosteine group. Biochemically tissue MDA, XO and SOD activities, light and electron microscopic findings were evaluated. Results: The erdosteine and α-tocoferol significantly reversed the effect of protein oxidation and lipid peroxidation induced by I/R shown by the decreased levels of MDA and XO activities. Both MDA and XO levels were found to be lower in group 6 compared to single agent treatment groups, and this was significantly different. All treatment groups showed increased SOD activity, which accounts for their oxidative properties. The mean Paller score of the combination treatment group (group 6) was lower than all groups except the sham group (3.67 ± 1.2), and this finding was statistically significant (0.05). Our results showed that the antioxidant pretreatment with α-tocopherol and erdosteine combination reduced lipid peroxidation of renal cellular membranes in a model of normothermic renal ischemia-reperfusion in rats. Combination of erdosteine and α-tocopherol has a synergistic effect of protection against oxidative processes. Long-term use of α-tocopherol seems to have a greater effect on the prevention of IR injury. However, further investigations are needed for the clinical applications of our findings. © 2009 Springer Science+Business Media, B.V.
Yazarlar (8)
1
Talat Yurdakul
2
Haluk Kulaksizoglu
3
Mehmet Mesut Pişkin
4
Mustafa Avunduk
5
Esra Ertemli
6
Hulagu Bariskaner
ORCID: 0000-0001-6600-4635
7
Sadik Büyükbaş
8
Volkan Kocabas
Anahtar Kelimeler
Antioxidants
Erdosteine
Ischemia-reperfusion injury
Kidney
α-Tocopherol
Kurumlar
Ankara Üniversitesi
Ankara Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
25
Atıf
8
Yazar
5
Anahtar Kelime