Scopus
YÖKSİS Eşleşti
Alterations in hepatic gene expressions of CYP2C11, CYP2C6V, and CYP2D3 enzymes in endotoxemic rats
Journal of Medical and Surgical Intensive Care Medicine · Ağustos 2017
YÖKSİS Kayıtları
Alterations in Hepatic Gene Expressions of CYP2C11, CYP2C6V, and CYP2D3 Enzymes in Endotoxemic Rats
journal of medical and surgical intensive care medicine · 2017 SCI-Expanded
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Makale Bilgileri
DergiJournal of Medical and Surgical Intensive Care Medicine
Yayın TarihiAğustos 2017
Cilt / Sayfa8 · 50-53
Scopus ID2-s2.0-85047871655
Özet
Objective: Human cytochrome P450 2C9 (CYP2C9), CYP2C19, and CYP2D6 (cor-responding to rat CYP2C11, CYP2C6V, and CYP2D3, respectively) are the major enzymes responsible for the metabolism of a wide range of drugs and chemicals. Changes in CYP efficiency are associated with disease susceptibility that can lead to drug toxicity or ineffective therapy. We aimed to examine the effects of lipopoly-saccharide (LPS)-induced endotoxemia on gene expressions of hepatic microsomal CYP enzymes in rats. Material and Methods: Male Wistar albino rats were allocated into two subgroups of control and LPS. Saline or LPS (10 mg/kg) of same volume (1 mL/kg) was intraperi-toneally (i.p.) injected into rats. After 4 hours, liver tissues were removed. Hepatic mRNA expressions of the CYP enzymes were quantitatively analyzed using real-time polymerase chain reaction (PCR). Results: Hepatic gene expressions of CYP2C11 and CYP2C6V decreased (3.35 and 2.25 fold, respectively) in the endotoxemic rats; however, CYP2D3 expression did not change. Conclusion: Our results revealed that endotoxemia changes CYP2C11-and CYP-2C6V-mediated drug metabolism. Therefore, drug dosage must be cautiously adjusted in the patients with endotoxemia and sepsis.
Yazarlar (7)
1
Erdem Kamil Ozer
2
Serkan Kurtgöz
3
Mustafa Tugrul Goktas
4
Ragıp Karaca
5
Metin Çalışkan
6
Hulagu Bariskaner
ORCID: 0000-0001-6600-4635
7
Alper Bektas Iskit
Anahtar Kelimeler
Cytochrome P450 enzymes
Endotoxemia
Gene expressions
Liver
Kurumlar
Ankara Yildirim Beyazit University
Ankara Turkey
Aydin Adnan Menderes University
Aydin Turkey
Hacettepe Üniversitesi
Ankara Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Süleyman Demirel University, Faculty of Medicine
Isparta Turkey
Metrikler
2
Atıf
7
Yazar
4
Anahtar Kelime