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The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey

Turkish Journal of Botany · Ağustos 2011

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YÖKSİS Kayıtları
The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey
TURKISH JOURNAL OF BOTANY · 2011 SCI-Expanded 4 atıf
PROFESÖR MUSTAFA YORGANCILAR →
The effect of thidiazuron on in vitro shoot development from endemic Astragalus cariensis in Turkey
Turkish Journal of Botany · 2011 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ EMİNE ATALAY →

Makale Bilgileri

DergiTurkish Journal of Botany
Yayın TarihiAğustos 2011
Cilt / Sayfa35 · 521-526
DOI10.3906/bot-1009-74
Scopus ID2-s2.0-80051778035
Erişim🔓 Açık Erişim
Özet Thidiazuron (TDZ) is a cytokinin-like substance that has often been used for shoot regeneration in recent years. We observed the effect of TDZ on callus formation and shoot regeneration from leaf and petiole explants of the endemic species Astragalus cariensis Boiss. Explants were cultured on Murashige and Skoog (MS) media including TDZ (in concentrations of 0.2, 0.4, or 0.6 mg/L) or in combination with α-naphthaleneacetic acid (NAA) (in concentrations of 0.2 or 1.0 mg/L). Significant variations in the frequency of callus formation and the morphogenesis of the callus were obtained depending on the explant type. Petiole explant was determined to be the best for callus formation, but calli from leaf explants were more responsive in terms of shoot formation. MS media including TDZ and NAA showed 100% callus formation, but shoot regeneration was enhanced by TDZ combined with a low concentration of NAA. The highest number of shoots, (15 per explant) was induced from leaf explants on MS medium with 0.4 mg/L TDZ and 0.2 mg/L NAA. Regenerated shoots were rooted in MS medium containing 0.5 mg/L indole-3-butyric acid (IBA). Th e rooted plantlets were acclimatised and produced normal plants.

Yazarlar (3)

1
Semiha Erışen
2
Emine Atalay
3
Mustafa Yorgancilar
ORCID: 0000-0003-4938-8547

Anahtar Kelimeler

Astragalus cariensis Callus Endemic Organogenesis Thidiazuron

Kurumlar

Selçuk Üniversitesi
Selçuklu Turkey

Metrikler

21
Atıf
3
Yazar
5
Anahtar Kelime

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MUSTAFA YORGANCILAR
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