Scopus
YÖKSİS Eşleşti
Investigation of glucose catabolism in hypoxic Mcf 7 breast cancer culture
Cytotechnology · Nisan 2021
YÖKSİS Kayıtları
Investigation of glucose catabolism in hypoxic Mcf 7 breast cancer culture
Cytotechnology · 2021 SCI-Expanded
ÖĞRETİM GÖREVLİSİ İREM BAYAR →
Makale Bilgileri
DergiCytotechnology
Yayın TarihiNisan 2021
Cilt / Sayfa73 · 217-232
Scopus ID2-s2.0-85101271304
Özet
Hypoxia plays an important role in tumor phenotype and progression and alters glycolysis, with changes in signaling pathways that develop in response to hypoxia. In this study, the effects of oxygen (normoxia/hypoxia) and of glucose levels on the glucose metabolism was investigated in MCF-7 cancer cells. Under either normoxia or hypoxia conditions, the cells were exposed to glucose at different concentrations (0, 5.5, 15 or 55 mM) for either 3, 6, 12, 24 or 48 h. In all groups, cell viability, levels of key enzymes reflecting glycolytic metabolism in cell lysates, glucose consumed in the medium and extracellular lactate levels and wound closure percentages were determined. In hypoxic cells, intracellular consumption of glucose, and extracellular lactate levels due to increased glucose concentration were observed to be higher (compared to normoxia) and as a result of prolonged exposure to hypoxia, cells were observed to develop resistance to the prolonged exposure to hypoxia. The number of glycolytic enzymes obtained at different levels proved that cells had different potential capacities and changing mechanisms for the metabolic needs of the cell depending on the glucose amount in the medium and time in adapting to the oxygen tension. This study showed that there was an important interaction between hypoxia and glucose metabolism in general, and it was concluded that metabolic processes activated by hypoxia could offer new therapeutic targets.
Yazarlar (2)
1
İrem Bayar
2
Ayşegül Bildik
Anahtar Kelimeler
Glucose
Glycolysis enzymes
Hypoxia
MCF-7
Normoxia
Kurumlar
Aydin Adnan Menderes University
Aydin Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
1
Atıf
2
Yazar
5
Anahtar Kelime