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In vitro and in vivo evaluation of levodopa-loaded nanoparticles for nose to brain delivery

Pharmaceutical Development and Technology · Temmuz 2020

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YÖKSİS Kayıtları
In vitro and in vivo evaluation of levodopa-loaded nanoparticles for nose to brain delivery
Pharmaceutical Development and Technology · 2020 SCI-Expanded
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Makale Bilgileri

DergiPharmaceutical Development and Technology
Yayın TarihiTemmuz 2020
Cilt / Sayfa25 · 735-747
Özet Parkinson’s disease (PD) is a neurodegenerative disease which is characterized by the loss of dopaminergic neurons in the brain. Levodopa is the drug of choice in the treatment of PD but it exhibits low oral bioavailability (30%) and very low brain uptake due to its extensive metabolism by aromatic amino acid decarboxylase in the peripheral circulation. Moreover, levodopa has psychic, gastrointestinal, and cardiovascular side effects, and most importantly, short and frequent stimulation of dopamine receptors lead to undesirable conditions such as dyskinesia over time. The challenges are to increase the therapeutic efficiency, the bioavailability and decreasing the unfavourable side effects of levodopa. Biocompatible nano-sized drug carriers could address these challenges at molecular level. For this purpose, levodopa-loaded Poly (lactide-co-glycolide) acid nanoparticles were prepared by double emulsion-solvent evaporation method for nose to brain drug delivery. Parameters such as homogenization speed, and external and internal phase content were modified to reach the highest loading efficiency. F1-1 coded formulation showed prolonged release up to 9 h. Carbodiimide method was used for surface modification studies of nanoparticles. The efficacy of the selected nanoparticle formulation has been demonstrated by in vivo experiments in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine induced PD model in mice.

Yazarlar (5)

1
Sema Arisoy
ORCID: 0000-0003-2798-1884
2
Tansel Comoglu
ORCID: 0000-0002-4221-5814
3
Deniz Onal
4
Ozbeyen Atalay
5
Bilge Pehlivanoglu

Anahtar Kelimeler

in vivo Parkinson disease model levodopa nanoparticles Nasal drug delivery Parkinson disease PLGA wheat germ aglutinine conjugation

Kurumlar

Ankara Üniversitesi
Ankara Turkey
Hacettepe Üniversitesi
Ankara Turkey
Inönü Üniversitesi
Malatya Turkey

Metrikler

64
Atıf
5
Yazar
6
Anahtar Kelime