Scopus
YÖKSİS DOI Eşleşti
SJR Q2
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
Molecular Biology Reports · Aralık 2024
YÖKSİS Kayıtları
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
Molecular Biology Reports · 2024 SCI-Expanded
Doç. Dr. DUDU ERKOÇ KAYA →
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
Molecular Biology Reports · 2024 SCI-Expanded
Prof. Dr. ESMA MENEVŞE →
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
Molecular Biology Reports · 2024 SCI-Expanded
Dr. Öğr. Üyesi DUYGU DURSUNOĞLU →
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
Molecular Biology Reports · 2024 EBSCO
Prof. Dr. HİLAL ARIKOĞLU →
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
Molecular Biology Reports · 2024 EBSCO
Dr. Öğr. Üyesi DUYGU DURSUNOĞLU →
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
Molecular Biology Reports · 2024 SCI-Expanded
Dr. Öğr. Üyesi DUYGU DURSUNOĞLU →
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2024 ISSN: 0301-4851 SCI-Expanded Q3
Prof. Dr. HÜSAMETTİN VATANSEV →
Investigation the effects of bee venom and H-dental-derived mesenchymal stem cells on non-small cell lung cancer cells (A549)
2024 ISSN: 0301-4851 SCI-Expanded Q3
Prof. Dr. BAHADIR ÖZTÜRK →
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
2024 ISSN: 0301-4851 SCI-Expanded Q3
Dr. Öğr. Üyesi DUYGU DURSUNOĞLU →
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
2024 ISSN: 0301-4851 SCI-Expanded Q3
Doç. Dr. DUDU ERKOÇ KAYA →
Juglone-ascorbate treatment enhances reactive oxygen species mediated mitochondrial apoptosis in pancreatic cancer
2024 ISSN: 0301-4851 SCI-Expanded Q3
Prof. Dr. ESMA MENEVŞE →
Investigation the effects of bee venom and H-dental-derived mesenchymal stem cells on non-small cell lung cancer cells (A549)
2024 ISSN: 0301-4851 SCI-Expanded Q3
Prof. Dr. HÜSAMETTİN VATANSEV →
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Makale Bilgileri
ISSN03014851
Yayın TarihiAralık 2024
Cilt / Sayfa51
Scopus ID2-s2.0-85185861030
Özet
Background: Treatment of Pancreatic Cancer (PC) is challenging due to its aggressiveness and acquired resistance to conventional chemotherapy and radiotherapy. Therefore, the discovery of new therapeutic agents and strategies is essential. Juglone, a naphthoquinone, is a secondary metabolite produced naturally in walnut-type trees having allelopathic features in its native environment. Juglone was shown to prevent cell proliferation and induce ROS-mediated mitochondrial apoptosis. Ascorbate with both antioxidant and oxidant features, shows selective cytotoxicity in cancer cells. Methods and results: In this study, we evaluated the anticancer effects of Juglone in combination with ascorbate in PANC-1 and BxPC-3 PC cells. The MTT assay was used to determine the IC<inf>50</inf> dose of Juglone with 1 mM NaAscorbate (Jug-NaAsc). Subsequently, the cells were treated with 5, 10, 15 and 20 µM Jug-NaAsc for 24 h. Apoptotic effects were evaluated by analyzing the following genes using qPCR; proapoptotic Bax, antiapoptotic Bcl-2 related to the mitochondrial apoptotic pathway and apoptosis inhibitor Birc5 (Survivin). Immunofluorescence analysis was performed using Annexin V-FITC in PC cells. As an antioxidant enzyme, Trx2 protein levels were determined by a commercial ELISA test kit. Jug-NaAsc treatment decreased the expressions of antiapoptotic genes Bcl-2 and Birc5 while the apoptotic gene Bax expression increased at all doses. Additionally, a dose-dependently increase of apoptosis according to immunofluorescence analysis and the decreases of Trx2 enzyme levels at all treatments in both cell lines supported gene expression results. Conclusion: Our results suggest that Juglone is a potential anticancer agent especially when combined with ascorbate.
Yazarlar (5)
1
Dudu Erkoc-Kaya
ORCID: 0000-0003-0114-6602
2
Hilal Arikoglu
ORCID: 0000-0002-6600-6603
3
Ebru Guclu
ORCID: 0000-0001-5330-6159
4
Duygu Dursunoǧlu
ORCID: 0000-0003-4414-8659
5
Esma Menevşe
Anahtar Kelimeler
Apoptosis
Juglone-Ascorbate
Pancreatic cancer
Prooxidant cancer therapy
Trx2
Kurumlar
Bozok Üniversitesi
Yozgat Turkey
Selçuk Tip Fakültesi
Konya Turkey
Scimago Dergi (ISSN Eşleşmesi)
Molecular Biology Reports
Q2
SJR Skoru0,743
H-Index103
YayıncıSpringer Science and Business Media B.V.
ÜlkeNetherlands
Genetics (Q2)
Medicine (miscellaneous) (Q2)
Molecular Biology (Q3)
Metrikler
6
Atıf
5
Yazar
5
Anahtar Kelime