Scopus
YÖKSİS Eşleşti
Preparation of Immobilized Metal Affinity Nanofiber for Globulin Depletion
Bionanoscience · Aralık 2020
YÖKSİS Kayıtları
Preparation of Immobilized Metal Affinity Nanofiber for Globulin Depletion
BIONANOSCIENCE · 2020 SCI-Expanded
ÖĞRETİM GÖREVLİSİ FATİH ÖZCAN →
Makale Bilgileri
DergiBionanoscience
Yayın TarihiAralık 2020
Cilt / Sayfa10 · 1084-1093
Scopus ID2-s2.0-85090847936
Özet
In this study, p-tert-butyl-calix[4]arene derivatives selectively were synthesized. Then, new nanofibers were obtained from this p-tert-butyl-Calix [4]arene amid derivatives by the electrospinning method. In this study, an IMA nanofiber system via iminodiacetic acid as ligand and metals as Cu<sup>2+</sup> for protein purification was developed. Copper (II) ions were chelated with IDA on nanofiber surfaces to prepare an immobilized metal affinity nanofiber (IMAN) for globulin depletion by the study of protein loading and release kinetics in various pH values. The loading and release of the globulin were monitored via fluorescence spectroscopy. The maximum amount of loaded protein onto 2.25 cm<sup>2</sup> of the CAL-IDA (CIDA) nanofiber surface was found to be 0.092 μg in 6.0 of pH for 60 min. Any release from protein-loaded nanofibers did not perform in any pH values. Loading of the protein to nanofibers was also clarified by SEM, TEM, EDX, AFM, and FT-IR analysis.
Yazarlar (4)
1
Othman Hameed
2
Fatih Ozcan
3
Seref Ertul
4
Esra Maltas Cagil
Anahtar Kelimeler
Calixarene nanofiber
Electrospin
Globulin
IMAN
Protein
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
1
Atıf
4
Yazar
5
Anahtar Kelime