Scopus
🔓 Açık Erişim
Bovine Fibroblast-Derived Extracellular Matrix Promotes the Growth and Preserves the Stemness of Bovine Stromal Cells during In Vitro Expansion
Journal of Functional Biomaterials · Nisan 2023
Makale Bilgileri
DergiJournal of Functional Biomaterials
Yayın TarihiNisan 2023
Cilt / Sayfa14
Scopus ID2-s2.0-85153951421
Erişim🔓 Açık Erişim
Özet
Cultivated meat is a fast-growing research field and an industry with great potential to overcome the limitations of traditional meat production. Cultivated meat utilizes cell culture and tissue engineering technologies to culture a vast number of cells in vitro and grow/assemble them into structures mimicking the muscle tissues of livestock animals. Stem cells with self-renewal and lineage-specific differentiation abilities have been considered one of the key cell sources for cultivated meats. However, the extensive in vitro culturing/expansion of stem cells results in a reduction in their abilities to proliferate and differentiate. Extracellular matrix (ECM) has been used as a culturing substrate to support cell expansion for cell-based therapies in regenerative medicine due to its resemblance to the native microenvironment of cells. In this study, the effect of the ECM on the expansion of bovine umbilical cord stromal cells (BUSC) in vitro was evaluated and characterized. BUSCs with multi-lineage differentiation potentials were isolated from bovine placental tissue. Decellularized ECM prepared from a confluent monolayer of bovine fibroblasts (BF) is free of cellular components but contains major ECM proteins such as fibronectin and type I collagen and ECM-associated growth factors. Expansion of BUSC on ECM for three passages (around three weeks) resulted in about 500-fold amplification, while cells were amplified less than 10-fold when cultured on standard tissue culture plates (TCP). Moreover, the presence of ECM reduced the requirement for serum in the culture medium. Importantly, the cells amplified on ECM retained their differentiation abilities better than cells cultured on TCP. The results of our study support the notion that monolayer cell-derived ECM may be a strategy to expand bovine cells in vitro effectively and efficiently.
Yazarlar (7)
1
Kathleen Lee
ORCID: 0009-0008-6456-8638
2
Anisha Jackson
ORCID: 0009-0004-3249-9851
3
Nikita John
4
Ryan Zhang
5
Derya Ozhava
ORCID: 0000-0003-4747-7490
6
Mohit Bhatia
7
Yong Mao
ORCID: 0000-0001-9268-4523
Anahtar Kelimeler
adipogenic differentiation
cell expansion
cultivated meat
extracellular matrix
stem cells
stemness
Kurumlar
Atelier Meats
Vancouver Canada
Rutgers University–New Brunswick
New Brunswick United States
Metrikler
7
Atıf
7
Yazar
6
Anahtar Kelime