Scopus
🔓 Açık Erişim YÖKSİS DOI Eşleşti
SJR Q3
Investigation of various virulence factors of Klebsiella pneumoniae strains isolated from nosocomial infections
Mikrobiyoloji Bulteni · Ekim 2017
YÖKSİS Kayıtları
Investigation of Various Virulence Factors of Klebsiellapneumoniae strains Isolated from Nosocomial Infections
MİKROBİYOLOJİ BÜLTENİ · 2017 SCI-Expanded
Prof. Dr. HATİCE TÜRK DAĞI →
Investigation of various Vilence Factors of Klebsiella pneumoniae strains isolated from nosocomial infections
Mikrobiyoloji Bülteni · 2017 SCI-Expanded
Prof. Dr. UĞUR ARSLAN →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Plasmodium falciparum ve Salmonella Typhi Koenfeksiyonu Bir Olgu Sunumu
2014 ISSN: 0374-9096 SCI-Expanded
Prof. Dr. ŞUA SÜMER →
Türkiye de İlk Kez Saptanan Hepatit B Virus Genotip H Enfeksiyonu Olgusu
2013 ISSN: 0374-9096 SCI-Expanded
Prof. Dr. ŞUA SÜMER →
A case of vancomycin resistant Leuconostoc meningitis
2008 ISSN: 0374-9096 SCI-Expanded 2 atıf
Prof. Dr. ENDER KÖKTEKİR →
In Vitro Synergistic Activity of Sulbactam in Combination with Imipenem Meropenem and Cefoperazone Against Carbapenem Resistant Acinetobacter baumannii Isolates
2014 ISSN: 03749096 SCI-Expanded 1 atıf
Prof. Dr. HATİCE TÜRK DAĞI →
Investigation of OXA type beta lactamases and PFGE patterns in Acinetobacter baumannii strains resistant to carbapenems
2014 ISSN: 0374-9096 SCI-Expanded
Prof. Dr. HATİCE TÜRK DAĞI →
Investigation of Various Virulence Factors of Klebsiellapneumoniae strains Isolated from Nosocomial Infections
2017 ISSN: 0374-9096 SCI-Expanded
Prof. Dr. HATİCE TÜRK DAĞI →
YMDD motif variants detected by Inno Lipa HBV DR assay in chronic hepatitis B patients during lamivudine therapy
2008 ISSN: 0374-9096 SCI-Expanded 3 atıf
Prof. Dr. ONUR URAL →
First Case of Hepatitis B Virus Genotype H Infection in Turkey
2013 ISSN: 0374-9096 SCI-Expanded
Prof. Dr. ONUR URAL →
Investigation of various Vilence Factors of Klebsiella pneumoniae strains isolated from nosocomial infections
2017 ISSN: 0374-9096 SCI-Expanded
Prof. Dr. UĞUR ARSLAN →
Streptococcus pyogenes İzolatlarının Virülans Faktörlerinin Araştırılması ve “Multiple Locus Variable Number Tandem Repeat Fingerprinting (MLVF)” Yöntemi ile Tiplendirilmesi
2018 ISSN: 0374-9096 SCI-Expanded
Prof. Dr. UĞUR ARSLAN →
Konya Bölgesinde Çeşitli Yaş Gruplarında Parvovirus B19 Seroprevalansının Araştırılması
2010 ISSN: 0374-9096 SCI-Expanded 1 atıf
Prof. Dr. HATİCE TÜRK DAĞI →
Trichophyton rubrum Klinik İzolatlarının Hemolitik Aktivitesi ve Antifungal İlaçlara İn vitro Duyarlılığının Saptanması
2011 ISSN: 0374-9096 SCI-Expanded 2 atıf
Prof. Dr. HATİCE TÜRK DAĞI →
Konya yöresinde 1998-2000 yılları arasında klinik örneklerden izole edilen Salmonella suşlarının serotipleri ve antibiyotik duyarlılıkları
2001 ISSN: 0374-9096 Endekste taranmıyor
Prof. Dr. UĞUR ARSLAN →
MOTAKK HBV DNA ve HCV RNA Dış Kalite Kontrol Ulusal Programı 2015-2016 Sonuçlarının Değerlendirilmesi
2018 ISSN: 0374-9096 SCI-Expanded
Prof. Dr. UĞUR ARSLAN →
Plasmodium falciparum and Salmonella Typhi Co-Infection: A Case Report
2014 ISSN: 0374-9096 SCI-Expanded
Prof. Dr. ONUR URAL →
Investigation of Antibiotic Resistance and Virulence Factors of Enterococcus faecium and Enterococcus faecalis Strains Isolatedfrom Clinical Samples
2020 ISSN: 0374-9096 SCI-Expanded
Prof. Dr. HATİCE TÜRK DAĞI →
The Epidemiology of Carbapenemases in Escherichia coli and Klebsiella pneumoniae Isolated in 2019 in Turkey
2021 ISSN: 0374-9096 SCI-Expanded Q4
Prof. Dr. HATİCE TÜRK DAĞI →
COVID-19 Tanısıyla Takip Edilen Hastalarda Annexin-1 (ANXA-1), Annexin-2 (ANXA-2) ve Kemik Morfogenetik Protein-7 (BMP-7) Serum Düzeyinin Değerlendirilmesi
2022 ISSN: 0374-9096 SCI-Expanded Q4
Prof. Dr. ŞUA SÜMER →
Makale Bilgileri
Dergi
Mikrobiyoloji Bulteni
ISSN03749096
Yayın TarihiEkim 2017
Cilt / Sayfa51 · 329-339
Scopus ID2-s2.0-85039917911
Erişim🔓 Açık Erişim
Özet
Klebsiella pneumoniae is an opportunistic pathogen that commonly affects immunosuppressed patients and causes nosocomial infections. K.pneumoniae has a variety of virulence factors, especially capsule polysaccharide, hypermucoviscosity (HV), fimbriae, toxins and determinants for iron acquisition. The aim of this study was to detect the virulence factors in K.pneumoniae strains isolated from nosocomial infections in two years. Fifty three K.pneumoniae strains isolated from the samples of patients with nosocomial infections In the Medical Microbiology Laboratory of Selcuk University Faculty of Medicine Hospital between 2011 and 2013 were included in the study. Identification and antimicrobial susceptibilities of the isolates were performed by VITEK 2 automatic system. Biofilm formation,a-hemolysin, capsule and HV were investigated by phenotypic methods. Polymerase chain reaction (PCR) was used to detect virulence genes encoding adhesins (fimH-1, mrkD, kpn, ycfM), siderophores (entB; enterobactin, iutA: aerobactin, ftp-7, irp-2, ybtS, fyuA: yersiniabactin, iroN; catechols receptor), protectees or invasins (rmpA, magA, traT) and toxins (hlyA, cnf1). Of the 53 K.pneumoniae isolates,! 2 (22.6%) were isolated from in patients of reanimation intensive care unit, 8 (15.1%) medical oncology, 7 (13.2%) newborn intensive care unit and 26 (49%) other clinics. The distribution of the isolates according to the samples was as follows: urine (n= 14), blood (n= 13), wound (n= 8), drainage fluid (n= 10), broncho-alveolar lavage (n= 7), and cerebrospinal fluid (n= 1). Isolates which were resistant to rneropenem were 5.7% and production of extended spectrum beta-lactamase (ESBL) was 71.7%. The capsule, biofilm formation, and HV were observed in 100%, 79.2%, and 1.9% of the isolates, respectively. Production of a-hemolysin was not detected in any of the isolates. The genes; entB (96.2%), ycfM (86.8%), and mrkD (83.0%) showed high prevalence. The other genes were detected in different ratios: fimH-1 (64.2%), fyuA (54.7%), kpn (49.1 %), ybtS (41.5%), /rp-7(41.5%), irp-2 (37.7%), traT01.3%) and iutA (5.7%). Virulence genes; iroN, rmpA, magA, hlyA and cnf-1 were not detected in any of the isolates. Enterobactin had the highest rate among siderophores, and ycfM and mrkD in adhesins. The capsule and biofilm formation were commonly found in the isolates. Hypermucoviscosity was only found in one isolate but associated genes were not detected. Alfa hemolysin production and hiyA gene were not determined. As a result, it seems that the basis of the pathogenicity of K.pneumoniae strains isolated from nosocomial infections are capsule, adhesins, enterobactin and ability of biofitm formation. There is a need for new studies for the continuous monitoring of toxin and invasion ability as well as antibiotic resistance in the control of hospital infection caused by K.pneumoniae.
Yazarlar (4)
1
Hatit Kus
2
Ugur Arslan
3
Hatice Türk Dağı
4
Duygu Findik
Anahtar Kelimeler
Hospital infection
Klebsiella pneumoniae
Virulence
Kurumlar
Konya Beyhekim State Hospital
Konya Turkey
Selçuk Tip Fakültesi
Konya Turkey
Scimago Dergi (ISSN Eşleşmesi)
Mikrobiyoloji Bulteni
Q3
SJR Skoru0,244
H-Index26
YayıncıAnkara Microbiology Society
ÜlkeTurkey
Immunology and Microbiology (miscellaneous) (Q3)
Infectious Diseases (Q3)
Medicine (miscellaneous) (Q4)
Microbiology (medical) (Q4)
Metrikler
33
Atıf
4
Yazar
3
Anahtar Kelime