Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
The epidemiology of carbapenemases in escherichia coli and klebsiella pneumoniae isolated in 2019 in Turkey
Mikrobiyoloji Bulteni · Ocak 2021
YÖKSİS Kayıtları
The Epidemiology of Carbapenemases in Escherichia coli and Klebsiella pneumoniae Isolated in 2019 in Turkey
MIKROBIYOLOJI BULTENI · 2021 SCI-Expanded
PROFESÖR HATİCE TÜRK DAĞI →
Makale Bilgileri
DergiMikrobiyoloji Bulteni
Yayın TarihiOcak 2021
Cilt / Sayfa55 · 1-16
Scopus ID2-s2.0-85101007782
Erişim🔓 Açık Erişim
Özet
Antibiotic resistance is one of the most important public health problem and one of the most critical steps in preventing resistance is the monitorization of the resistance. Local, regional and global monitoring enables the spread of antibiotic resistance to be understood more clearly. In this study, it was aimed to evaluate the results of the pilot study for the establishment of molecular-based carbapenem surveillance system in Escherichia coli and Klebsiella pneumoniae isolates and to investigate the carbapenemase epidemiology in Turkey. Hospitals (n= 28) from 26 different statistical level II regions from Turkey were included in the study. The hospitals participated in the study submitted ten carbapenem susceptible and ten carbapenem resistant E.coli and K.pneumoniae isolates to our laboratory that were isolated in two different periiods of six-month either between 1 March-31 August or 1 April-30 September 2019. A total of 509 isolates were collected from 26 of the 28 participating hospitals in the study. Isolates were identified by matrix assisted laser desorptionization-time of flight mass spectrophotometry (MALDI TOF MS) (Bruker Daltonics, Germany) method and antibiotic susceptibility tests for imipenem, meropenem and colistin were studied by broth microdilution. Moreover, susceptibilities to amikacin, amoxicillin-clavulanic acid, ampicillin, aztreonam, cefepime, cefotaxime, ceftazidime, ciprofloxacin, ertapenem, gentamicin, piperacillin-tazobactam, tobramycin and trimethoprim-sulfamethoxazole were determined by disc diffusion method. The resistance genes were investigated in isolates which were found to be phenotypically resistant to carbapenem and colistin, in house method was used to investigate carbapenemase genes and a commercial colistin resistant real-time PCR kit (Biospeedy, Turkey) was used for colistin resistance genes. In total, 493 of the 509 isolates collected from hospitals were identified as E.coli (25.7%, n= 127) and K.pneumoniae (74.3%, n= 366) and included in the study. It was determined that 31% of the isolates evaluated were from community-acquired infections and 69% were either from healthcare-associated infections or from colonization sites. Among the tested isolates, 248 (50.3%) were susceptible to carbapenems and 245 (49.7%) were resistant. The types of carbapenemases in carbapenemase-producing isolates were OXA-48 (52.2%), KPC (16.1%), NDM-1 (15%), OXA-48 + NDM-1 (12.6%), KPC + NDM-1 (2.8%) and VIM (0.5%) and OXA-48+VIM (0.5%). Resistance to colistin was detected in 23.3% of the isolates but mcr1-8 genes were not detected. It was found that all colistin resistant isolates are resistant to at least one of the carbapenems. The importance of a molecular-based antimicrobial resistance surveillance system in our country was demonstrated with this pilot study. It is thought that continuous monitoring of these epidemiological features will contribute to the management of infections due to carbapenemase-producing organisms.
Yazarlar (36)
1
Serap Süzük Yildiz
2
Hüsniye Şimşek
3
Zekiye Bakkaloglu
4
Yasemin Numanoǧlu Çevik
5
Can Hüseyin Hekimoǧlu
6
Selçuk Kiliç
7
Emine Alp Meşe
8
Alper Akçali
9
Ayşe Ulusoy Karaca
10
Banu Bayraktar
11
Canan Eryildiz
12
Cem Çelik
13
Devrim Dündar
14
Ebru Evren
15
Eda Demirkan
16
Esra Özkaya
17
Fatma Baǧci
18
Fikriye Milleti Sezgin
19
Filiz Kibar
20
Hatice Türk Dağı
21
Hüseyin Güdücüoğlu
22
Ipek Mumcuoǧlu
23
Mahmut Celalettin Üner
24
Melahat Gürbüz
25
Murat Telli
26
Mustafa Z. Bakici
27
Nergis Aşgin
28
Osman Sezer Cirit
29
Pervin Özlem Balci
30
Riza Adaleti
31
Sebahat Aksaray
32
Senem Akgül
33
Şöhret Aydemir
ORCID: 0000-0001-8354-9100
34
Yeşim Çekin
35
Yücel Duman
36
Zeynep Ceren Karahan
Anahtar Kelimeler
Carbapenemase
E.coli
K.pneumoniae
Surveillance system
Kurumlar
Ankara Şehir Hastanesi
Ankara Turkey
Ankara Üniversitesi
Ankara Turkey
Antalya Eğitim ve Araştırma Hastanesi
Antalya Turkey
Aydin Adnan Menderes University
Aydin Turkey
Çukurova Üniversitesi Tip Fakültesi
Adana Turkey
Cumhuriyet Üniversitesi Tip Fakültesi
Sivas Turkey
Dr. Münif Islamoǧlu Kastamonu Devlet Hastanesi
Kastamonu Turkey
Ege University Medical School
Izmir Turkey
Erzurum Bölge Eğitim ve Araştırma Hastanesi
Erzurum Turkey
Gaziantep Dr. Ersin Arslan Eğitim ve Araştırma Hastanesi
Gaziantep Turkey
Hatay Antakya Devlet Hastanesi
Turkey
Haydarpasa Numune Egitim ve Arastýrma Hastanesi
Istanbul Turkey
Inönü Üniversitesi Tip Fakültesi
Malatya Turkey
Karadeniz Teknik Üniversitesi Tip Fakültesi
Trabzon Turkey
Kırşehir Ahi Evran Üniversitesi
Kirsehir Turkey
Kocaeli Üniversitesi
İzmit Turkey
Manisa Devlet Hastanesi
Manisa Turkey
Mardin Devlet Hastanesi
Mardin Turkey
Mikrobiyoloji Laboratuvari
Karabuk Turkey
On Sekiz Mart University
Canakkale Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Şevket Yılmaz Eğitim ve Araştırma Hastanesi
Bursa Turkey
Şişli Hamidiye Etfal Eğitim ve Araştırma Hastanesi
Istanbul Turkey
T.C. Sağlık Bakanlığı,
Ankara Turkey
Tokat Devlet Hastanesi
Tokat Turkey
Trakya University, Faculty of Medicine
Edirne Turkey
Üniversitesi Tip Fakültesi
Sivas Turkey
Van Yüzüncü Yıl Üniversitesi
Van Turkey
Metrikler
29
Atıf
36
Yazar
4
Anahtar Kelime