Scopus
Smartphone-integrated NIR fluorescent probe for rapid and sensitive alkaline phosphatase detection in serum and living cells
Talanta · Mayıs 2026
Makale Bilgileri
DergiTalanta
Yayın TarihiMayıs 2026
Cilt / Sayfa302
Scopus ID2-s2.0-105027938457
Özet
Alkaline phosphatase (ALP) is a clinically significant hydrolase enzyme involved in various physiological and pathological processes, making its accurate detection vital for disease diagnosis and monitoring. In this study, we report a new near-infrared (NIR) fluorescent probe based on a triphenylamine–dicyanoisophorone scaffold functionalized with a phosphate group for the rapid and sensitive detection of ALP activity. The probe is designed with a donor–acceptor (D–A) structure, in which triphenylamine serves as a strong electron donor and dicyanoisophorone as an efficient electron acceptor, promoting intramolecular charge transfer (ICT) upon enzymatic activation. Initially, the non-fluorescent probe exhibits a robust “turn-off/on” response upon ALP-mediated dephosphorylation, resulting in a strong NIR emission at 650 nm. The system demonstrates excellent sensitivity with a detection limit of 0.048 U/L and a rapid response time of approximately 8.0 min-outperforming many previously reported ALP sensors. The probe shows high selectivity toward ALP over various biologically relevant metal ions, anions, and amino acids. Furthermore, a smartphone-based RGB analysis enables low-cost and user-friendly quantification of ALP levels. Molecular docking studies support the probe's strong binding affinity to ALP, with a calculated binding energy of −8.2 kcal/mol. Importantly, the probe was successfully applied for the detection of ALP in human serum samples, with results consistent with the standard p NPP assay, confirming its potential for clinical applications. Owing to its superior analytical performance, biocompatibility, and point-of-care potential, this probe represents a promising tool for real-time ALP detection in biomedical and clinical applications.
Yazarlar (4)
1
Alev Oguz
2
Serkan Erdemir
ORCID: 0000-0003-0790-0549
3
Sait Malkondu
4
Mehmet Oguz
Anahtar Kelimeler
ALP
Bioimaging
ICT
Isophorone
NIR fluorescence
Triphenylamine
Kurumlar
Giresun Üniversitesi
Giresun Turkey
Selçuk Üniversitesi
Selçuklu Turkey