Scopus
🔓 Açık Erişim
Unleashing the biological power and chemical profile of Paracaryum hedgei extracts based on chromatographic, in vitro, and bioinformatic tools
Biochemistry and Biophysics Reports · Mart 2026
Makale Bilgileri
DergiBiochemistry and Biophysics Reports
Yayın TarihiMart 2026
Cilt / Sayfa45
Scopus ID2-s2.0-105024903876
Erişim🔓 Açık Erişim
Özet
Studies evaluating the chemical constituents and pharmacological potential of Paracaryum species (family Boraginaceae) were limited. The current study was designed to investigate, for the first time, the chemical profile, antioxidant, enzyme-inhibitory, and cytotoxic properties of P. hedgei Aytaç & R.R. Mill. Roots extracts recorded the highest total phenolic content (24.72–91.16 mg GAE/g). About 33 compounds were identified, and the aerial parts extracts were dominated by rosmarinic acid, rutin, and sagerinic acid. In general, the root extracts exhibited greater antioxidant activity than the extracts from the aerial parts. Among the solvents, The 70 % EtOH extract of roots showed the highest antiradical (DPPH: 207.90 mg TE/g; ABTS: 238.08 mg TE/g), ion-reducing (FRAP: 384.99 mg TE/g; CUPRAC: 615.27 mg TE/g), and total antioxidant activities (3.21 mmol TE/g), whereas the EtOAc and aqueous extracts from the aerial parts demonstrated the best chelating capacity. The enzyme inhibition varied according to the plant parts examined and the solvents employed for extraction. The EtOAc extract of the roots exerted the highest inhibitory effect towards the human carbonic anhydrase II (IC<inf>50</inf>: 2.32 μg/ml). The cytotoxicity of the extracts was tested against three cancer cell lines (HELA, A549, and HCT-116) and one healthy cell line (HEK-293). Specifically, the EtOAC extracts showed cytotoxic effects on HELA cells sourced from aerial parts (IC<inf>50</inf>: 106.30 μg/ml) and on HCT-116 cells sourced from roots (IC<inf>50</inf>: 96.82 μg/ml). Molecular docking results provided additional support for the enzyme inhibition capabilities of the extracts. Combined network pharmacology and molecular docking analyses revealed that phenolic acids from P. hedgei exert anti-cancer effects on cervical and colorectal adenocarcinoma by modulating key pathways, including retinoic acid metabolism, steroid biosynthesis, and inflammatory responses. These findings can provide a scientific starting point for the pharmaceutical potential of P. hedgei , and it can be considered a valuable source of natural bioactive compounds for functional nutraceutical and pharmaceutical applications.
Yazarlar (12)
1
Enver Saka
2
Sakina Yagi
3
Bengusu Hacer Akgul
4
E. J. Llorent-Martínez
5
Evren Yildiztugay
ORCID: 0000-0002-4675-2027
6
Ismail Koyuncu
7
Meltem Cayci
ORCID: 0000-0002-3401-9186
8
İsmail Yapıcı
9
Gülçin
10
Yimao Wu
11
Meng Yao Li
ORCID: 0000-0002-7054-448X
12
Gokhan Zengin
ORCID: 0000-0001-6548-7823
Anahtar Kelimeler
Carbonic anhydrase
Network pharmacology
Paracaryum hedgei
Radical scavenging
Salvianolic acid
Kurumlar
Atatürk Üniversitesi
Erzurum Turkey
Central Hospital of Shanghai Jiading District
Shanghai China
Harran Üniversitesi
Sanliurfa Turkey
Harran University, Faculty of Medicine
Sanliurfa Turkey
Khartoum University
Khartoum Sudan
Renji Hospital
Shanghai China
Selçuk Üniversitesi
Selçuklu Turkey
Universidad de Jaén
Jaen Spain