Scopus
🔓 Açık Erişim YÖKSİS DOI Eşleşti
Phytochemical profile and biological properties of colchicum triphyllum (Meadow Saffron)
Foods · Nisan 2020
YÖKSİS Kayıtları
Phytochemical Profile and Biological Properties of Colchicum triphyllum (Meadow Saffron)
Foods · 2020 SCI-Expanded
Prof. Dr. GÖKHAN ZENGİN →
Phytochemical Profile and Biological Properties of Colchicum triphyllum (Meadow Saffron)
Foods · 2020 SCI-Expanded
Prof. Dr. EVREN YILDIZTUGAY →
Makale Bilgileri
Dergi
Foods
Yayın TarihiNisan 2020
Cilt / Sayfa9
Scopus ID2-s2.0-85083319946
Erişim🔓 Açık Erişim
Özet
In this work, the phytochemical profile and the biological properties of Colchicum triphyllum (an unexplored Turkish cultivar belonging to Colchicaceae) have been comprehensively investigated for the first time. Herein, we focused on the evaluation of the in vitro antioxidant and enzyme inhibitory effects of flower, tuber, and leaf extracts, obtained using different extraction methods, namely maceration (both aqueous and methanolic), infusion, and Soxhlet. Besides, the complete phenolic and alkaloid untargeted metabolomic profiling of the different extracts was investigated. In this regard, ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) allowed us to putatively annotate 285 compounds when considering the different matrix extracts, including mainly alkaloids, flavonoids, lignans, phenolic acids, and tyrosol equivalents. The most abundant polyphenols were flavonoids (119 compounds), while colchicine, demecolcine, and lumicolchicine isomers were some of the most widespread alkaloids in each extract analyzed. In addition, our findings showed that C. triphyllum tuber extracts were a superior source of both total alkaloids and total polyphenols, being on average 2.89 and 10.41 mg/g, respectively. Multivariate statistics following metabolomics allowed for the detection of those compounds most affected by the different extraction methods. Overall, C. triphyllum leaf extracts showed a strong in vitro antioxidant capacity, in terms of cupric reducing antioxidant power (CUPRAC; on average 96.45 mg Trolox Equivalents (TE)/g) and ferric reducing antioxidant power (FRAP) reducing power (on average 66.86 mg TE/g). Interestingly, each C. triphyllum methanolic extract analyzed (i.e., from tuber, leaf, and flower) was active against the tyrosinase in terms of inhibition, recording the higher values for methanolic macerated leaves (i.e., 125.78 mg kojic acid equivalent (KAE)/g). On the other hand, moderate inhibitory activities were observed against AChE and ff-amylase. Strong correlations (p < 0.01) were also observed between the phytochemical profiles and the biological activities determined. Therefore, our findings highlighted, for the first time, the potential of C. triphhyllum extracts in food and pharmaceutical applications.
Yazarlar (8)
1
Biancamaria Senizza
2
Gabriele Rocchetti
3
Murat Ali Okur
4
Gokhan Zengin
ORCID: 0000-0001-6548-7823
5
Evren Yildiztugay
ORCID: 0000-0002-4675-2027
6
Gunes Ak
7
Domenico Montesano
ORCID: 0000-0002-8857-2091
8
Luigi Lucini
ORCID: 0000-0002-5133-9464
Anahtar Kelimeler
Antioxidants
Bioactive compounds
Extraction methods
Meadow saffron
Metabolomics
UHPLC-QTOF-mass spectrometry
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Università Cattolica del Sacro Cuore, Campus di Piacenza e Cremona
Piacenza Italy
Università degli Studi di Perugia
Perugia Italy
Metrikler
25
Atıf
8
Yazar
6
Anahtar Kelime