Scopus
YÖKSİS Eşleşti
Comprehensive evaluation of two Astragalus species (A. campylosema and A. hirsutus) based on biological, toxicological properties and chemical profiling
Food and Chemical Toxicology · Ağustos 2021
YÖKSİS Kayıtları
Comprehensive evaluation of two Astragalus species (A. campylosema and A. hirsutus) based on biological, toxicological properties and chemical profiling
Food and Chemical Toxicology · 2021 SCI-Expanded
PROFESÖR EVREN YILDIZTUGAY →
Makale Bilgileri
DergiFood and Chemical Toxicology
Yayın TarihiAğustos 2021
Cilt / Sayfa154
Scopus ID2-s2.0-85107931908
Özet
Astragalus L. (Fabaceae) is an important genus with numerous species having various traditional medicinal uses making them of interest for scientific investigations to ascertain their therapeutic benefits. In the present study, the quantitative polyphenolic profiles of methanolic extracts from different parts (leaves, flowers, and roots) of two endemic Astragalus species growing in Turkey, i.e. A. campylosema Boiss. and A. hirsutus Vahl were determined, along with their antioxidant and enzyme inhibitory properties. A. campylosema and A. hirsutus extracts showed varying total phenolic (25.80–40.60 and18.59–29.46 mg GAE/g, respectively) and total flavonoid (11.21–105.91 and 16.06–131.91 mg RE/g, respectively) contents. HPLC-MS/MS revealed rutin to be the predominant phenolic compound in all the extracts of A. campylosema and leaf extract of A. hirsutus (133.53–752.42 μg g−1), while hyperoside was the major one in the flower and root extracts of A. hirsutus (2014.07 and 123.13 μg g−1, respectively). In DPPH and ABTS assays, radical scavenging capacity was demonstrated by all extracts of A. campylosema (47.13–48.10 and 87.03–115.36 mg TE/g, respectively) and A. hirsutus (17.82–38.67 and 47.84–57.29 mg TE/g, respectively). Reducing activity was also displayed by the extracts in CUPRAC and FRAP assays (A. campylosema: 83.06–135.20 and 59.15–90.19 mg TE/g, respectively; A. hirsutus: 53.02–83.42 and 31.25–43.25 mg TE/g, respectively). All extracts were also found to act as metal chelators (12.32–21.45 mg EDTAE/g) and exhibited total antioxidant capacity ranging from 1.16 to 1.60 mmol TE/g, in phosphomolybdenum assay. Acetyl- and butyryl-cholinesterase inhibitory effects were observed by all the extracts of the two species (1.56–4.99 mg GALAE/g). Anti-hyperpigmentation potential by inhibiting tyrosinase (54.55–67.35 mg KAE/g) was reported as well. Carbohydrate hydrolyzing enzymes, amylase and glucosidase were also inhibited (0.22–1.03 mmol ACAE/g). Overall, A. campylosema extracts showed relatively better antioxidant and enzyme inhibitory potentials compared to A. hirsutus extracts. Strikingly, A. hirsutus extracts was found to have higher AGE inhibition activity than A. campylosema. Although the cytotoxic effect of three different organs obtained from A. campylosema and A. hirsutus increased depending on the dose (from 10 to 200 μg/mL), it was found that both plant extracts did not show a genotoxic effect at the highest concentration of 200 μg/mL. Indeed, data amassed from this current scientific work showed the two selected Astragalus species to be rich in bioactive polyphenols that could be responsible for the various pharmacological activities and hence demands to be further explored for their possible applications as natural health promoting agents.
Yazarlar (11)
1
Inci Kurt-Celep
2
Gokhan Zengin
ORCID: 0000-0001-6548-7823
3
Kouadio Ibrahime Sinan
4
Gunes Ak
5
Fevzi Elbasan
6
Evren Yildiztugay
ORCID: 0000-0002-4675-2027
7
Filippo Maggi
ORCID: 0000-0003-1375-4744
8
Giovanni Caprioli
ORCID: 0000-0002-5530-877X
9
Simone Angeloni
10
Sharmeen Jugreet
11
Mohamad Fawzi Mahomoodally
Anahtar Kelimeler
Antioxidant
Astragalus species
Cytotoxicity
Enzymes inhibition
Flavonoids
Genotoxicity
HeLa cell Line
Phenolics
Kurumlar
International Hub for Coffee Research and Innovation
Belforte del Chienti (MC) Italy
Selçuk Üniversitesi
Selçuklu Turkey
Università degli Studi di Camerino
Camerino Italy
University of Mauritius
Reduit Mauritius
Yeditepe University
Istanbul Turkey
Metrikler
14
Atıf
11
Yazar
8
Anahtar Kelime