Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Selectivity Tuning by Natural Deep Eutectic Solvents (NADESs) for Extraction of Bioactive Compounds from Cytinus hypocistis—Studies of Antioxidative, Enzyme-Inhibitive Properties and LC-MS Profiles
Molecules · Eylül 2022
YÖKSİS Kayıtları
Selectivity tuning by natural deep eutectic solvents (NADESs) for extraction of bioactive compounds from Cytinus hypocistis—studies of antioxidative, enzyme-inhibitive properties and LC-MS profiles
MOLECULES · 2022 SCI-Expanded
PROFESÖR EVREN YILDIZTUGAY →
Makale Bilgileri
DergiMolecules
Yayın TarihiEylül 2022
Cilt / Sayfa27
Scopus ID2-s2.0-85138437842
Erişim🔓 Açık Erişim
Özet
In the present study, the extracts of Cytinus hypocistis (L.) L using both traditional solvents (hexane, ethyl acetate, dichloromethane, ethanol, ethanol/water, and water) and natural deep eutectic solvents (NADESs) were investigated in terms of their total polyphenolic contents and antioxidant and enzyme-inhibitive properties. The extracts were found to possess total phenolic and total flavonoid contents in the ranges of 26.47–186.13 mg GAE/g and 0.68–12.55 mg RE/g, respectively. Higher total phenolic contents were obtained for NADES extracts. Compositional differences were reported in relation to antioxidant potential studied by several assays (DPPH: 70.19–939.35 mg TE/g, ABTS: 172.56–4026.50 mg TE/g; CUPRAC: 97.41–1730.38 mg TE/g, FRAP: 84.11–1534.85 mg TE/g). Application of NADESs (choline chloride—urea 1:2, a so-called Reline) allowed one to obtain the highest number of extracts having antioxidant potential in the radical scavenging and reducing assays. NADES-B (protonated by HCl L-proline-xylitol 5:1) was the only extractant from the studied solvents that isolated a specific fraction without chelating activity. Reline extract exhibited the highest acetylcholinesterase inhibition compared to NADES-B and NADES-C (protonated by H2SO4 L-proline-xylitol 5:1) extracts, which showed no inhibition. The NADES extracts were observed to have higher tyrosinase inhibitory properties compared to extracts obtained by traditional organic solvents. Furthermore, the NADES extracts were relatively better inhibitors of the diabetic enzymes. These findings provided an interesting comparison in terms of total polyphenolic content yields, antioxidant and enzyme inhibitory properties (cholinesterase, amylase, glucosidase, and tyrosinase) between traditional solvent extracts and NADES extracts, used as an alternative. While the organic solvents showed better antioxidant activity, the NADES extracts were found to have some other improved properties, such as higher total phenolic content and enzyme-inhibiting properties, suggesting functional prospects for their use in phytonutrient extraction and fractionation. The obtained results could also be used to give a broad overview of the different biological potentials of C. hypocistis.
Yazarlar (11)
1
Gokhan Zengin
ORCID: 0000-0001-6548-7823
2
María de la Luz Cádiz-Gurrea
3
Álvaro Fernández-Ochoa
4
Francisco Javier Leyva-Jiménez
5
Antonio Segura Carretero
6
Malwina Momotko
7
Evren Yildiztugay
ORCID: 0000-0002-4675-2027
8
Refik Karatas
9
Sharmeen Jugreet
10
Mohamad Fawzi Mahomoodally
11
Grzegorz Boczkaj
ORCID: 0000-0002-5874-7591
Anahtar Kelimeler
Alzheimer cholinesterase inhibitors
antioxidants
enzyme inhibition
functional food
NADES
natural medicine
total polyphenolic content
Kurumlar
Gdańsk University of Technology
Gdansk Poland
North-West University
Potchefstroom South Africa
Saveetha Dental College And Hospitals
Chennai India
Selçuk Üniversitesi
Selçuklu Turkey
Universidad de Castilla-La Mancha
Ciudad Real Spain
Universidad de Granada, Facultad de Ciencias
Granada Spain
University of Mauritius
Reduit Mauritius
Metrikler
14
Atıf
11
Yazar
7
Anahtar Kelime