Scopus
YÖKSİS Eşleşti
Evaluation of AFP III-Conjugated Fe3O4 Nanoparticles on Bull Sperm Cryopreservation
Reproduction in Domestic Animals · Ekim 2025
YÖKSİS Kayıtları
Evaluation of AFP III‐Conjugated Fe3O4 Nanoparticles on Bull Sperm Cryopreservation
Reproduction in Domestic Animals · 2025 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUSTAFA BODU →
Makale Bilgileri
DergiReproduction in Domestic Animals
Yayın TarihiEkim 2025
Cilt / Sayfa60
Scopus ID2-s2.0-105017832543
Özet
Antifreeze proteins are naturally occurring proteins that are known for their ability to inhibit ice formation. Although effective in nature, they have failed to produce the expected results in cryobiological studies. However, the lack of a significant effect when used alone has raised the following question: Could conjugation with Fe<inf>3</inf>O<inf>4</inf> nanoparticles (Fe<inf>3</inf>O<inf>4</inf> NPs), which are frequently used in drug delivery studies, enable closer interaction with spermatozoa and provide cumulative protection? To address this question, bull spermatozoa were used. First, Fe<inf>3</inf>O<inf>4</inf> NPs (~6 nm) and AFP III-conjugated Fe<inf>3</inf>O<inf>4</inf> NPs (AFP III@Fe<inf>3</inf>O<inf>4</inf> NPs) were synthesised and characterised by dynamic light scattering, zeta potential analysis, and FTIR spectroscopy to confirm the conjugation. Then, sperm samples collected from five different Holstein bulls for five replications were diluted with AndroMed extender and divided into five groups: control, Fe<inf>3</inf>O<inf>4</inf> NPs only, AFP III only (0.1 and 0.5 μg/mL), and AFP III@Fe<inf>3</inf>O<inf>4</inf> NPs (0.042 and 0.075 μg/mL). After cryopreservation, spermatological parameters and DNA condensation were analysed. The AFP III@Fe<inf>3</inf>O<inf>4</inf> NPs (0.075 μg/mL) exhibited significantly higher motility and DNA integrity compared to the other treatment groups (p < 0.05). However, no statistically significant differences were observed in mitochondrial membrane potential, membrane, and acrosomal integrity. Analyses of motility and DNA damage supported the protective effect of the AFP III@Fe<inf>3</inf>O<inf>4</inf> NPs 0.075 μg/mL formulation. Thus, we have demonstrated the applicability of AFP III@Fe<inf>3</inf>O<inf>4</inf> NPs, for the first time, in bull sperm cryopreservation and have introduced a novel nano-biotechnological approach for improving the success of assisted reproductive technologies in livestock.
Yazarlar (9)
1
Ali Erdem Öztürk
2
Seyma Dadi
3
Mustafa Bodu
ORCID: 0000-0002-8432-7347
4
Oya Korkmaz
5
Yunus Emre Atay
6
Serpil Sariözkan
7
Ramazan Uzen
ORCID: 0000-0002-2208-1361
8
Halil Aydin Simsek
9
Ismail Ocsoy
ORCID: 0000-0002-5991-3934
Anahtar Kelimeler
AFPIII
bull
cryopreservation
Fe3O4 NP
sperm
Kurumlar
Erciyes Üniversitesi
Kayseri Turkey
Malatya Turgut Ozal University
Malatya Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Sivas Science and Technology University
Sivas Turkey
Super Genetic Sperm Production Farm
Konya Turkey