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Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen
Scientific Reports · Aralık 2025
YÖKSİS Kayıtları
Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen
Scientific Reports · 2025 SSCI
DOÇENT DURMUŞ HATİPOĞLU →
Makale Bilgileri
DergiScientific Reports
Yayın TarihiAralık 2025
Cilt / Sayfa15
Scopus ID2-s2.0-105017643477
Erişim🔓 Açık Erişim
Özet
The SARS-CoV-2 pandemic has led to major advances in mRNA vaccine development. This study aimed to develop a cationic lipid nanoparticle (LNP) coated mRNA vaccine targeting the receptor binding domain (RBD) against the SARS-CoV-2 virus and adding valine to the N-terminal end to increase its stability, and to comprehensively characterize the physical, thermal, microscopic and biological properties of vaccine. The vaccine was designed in silico and valine-added S-RBD molecule was generated by IVT. The LNP coated mRNA vaccine was evaluated by spectroscopic (UV–vis, FT-IR, Raman), thermodynamic (TGA/DSC), microscopic (STEM, AFM), size/potential (DLS) and surface tension analysis methods. The developed vaccine was analyzed for cytotoxicity (MTT) and pro/anti-inflammatory gene expression (qPCR). Spectroscopic analyses revealed distinct RNA and lipid peaks. Thermodynamic analyses revealed complete mass loss for mRNA and LNP at 136.2 °C and 85.7 °C, respectively. STEM analysis revealed that mRNA was uniformly coated with LNP, and its size was < 100 nm, and Ra/Rz values were 26.56/34.29 nm, respectively. Zeta potentials ranged from 0 to − 30 mV, and surface tensions were 33.8–38.0 mN/m, respectively. MTT analysis showed that the vaccine was not cytotoxic. qPCR analysis, significant increases were detected in IFNA1, IFNB1 and TNF gene expressions, but no significant increase was detected in IL6. Bioinformatic analyses revealed increased stability in mRNA structure and more robust pseudoknot formations after valine addition. Our study is the first study in which a uniquely designed valine-added mRNA vaccine coated with LNP targeting the RBD antigen of SARS-CoV-2 was developed and comprehensively characterized.
Yazarlar (9)
1
Tugce Duran
ORCID: 0000-0002-7353-4527
2
Naci Cine
ORCID: 0000-0001-9063-1073
3
Mehmet Ali Karaselek
4
Nadir Gul
ORCID: 0000-0003-1259-4910
5
Serkan Kuccukturk
6
Durmus Hatipoglu
ORCID: 0000-0003-3790-7821
7
Mehmet Burak Ates
ORCID: 0000-0003-1297-426X
8
Ümran Çalişkan
9
Nadir Koçak
ORCID: 0000-0002-1104-1292
Anahtar Kelimeler
Characterization
S-RBD
Vaccine
Valine
Kurumlar
Karamanoğlu Mehmetbey Üniversitesi
Karaman Turkey
Karatay Üniversitesi
Konya Turkey
Kocaeli Üniversitesi
İzmit Turkey
Malta Life Sciences Park
San Gwann Malta
Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Tip Fakültesi
Konya Turkey
Selçuk Üniversitesi
Selçuklu Turkey